Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5468865..5469490 Replicon chromosome
Accession NZ_CP089385
Organism Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag LUX40_RS26225 Protein ID WP_008807903.1
Coordinates 5468865..5469248 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LUX40_RS26230 Protein ID WP_004150355.1
Coordinates 5469248..5469490 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX40_RS26210 (LUX40_26210) 5466231..5467133 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LUX40_RS26215 (LUX40_26215) 5467130..5467765 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
LUX40_RS26220 (LUX40_26220) 5467762..5468691 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LUX40_RS26225 (LUX40_26225) 5468865..5469248 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
LUX40_RS26230 (LUX40_26230) 5469248..5469490 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LUX40_RS26235 (LUX40_26235) 5469695..5470612 + 918 WP_016162034.1 alpha/beta hydrolase -
LUX40_RS26240 (LUX40_26240) 5470626..5471567 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LUX40_RS26245 (LUX40_26245) 5471612..5472049 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
LUX40_RS26250 (LUX40_26250) 5472046..5472906 - 861 WP_008807907.1 virulence factor BrkB family protein -
LUX40_RS26255 (LUX40_26255) 5472900..5473499 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T228149 WP_008807903.1 NZ_CP089385:c5469248-5468865 [Klebsiella variicola subsp. variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T228149 NZ_CP123365:c2974737-2974330 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT228149 WP_004150355.1 NZ_CP089385:c5469490-5469248 [Klebsiella variicola subsp. variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT228149 NZ_CP123365:c2974984-2974718 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References