Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4235308..4235927 Replicon chromosome
Accession NZ_CP089385
Organism Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LUX40_RS20430 Protein ID WP_002892050.1
Coordinates 4235709..4235927 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag LUX40_RS20425 Protein ID WP_008805436.1
Coordinates 4235308..4235682 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX40_RS20415 (LUX40_20415) 4230463..4231656 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LUX40_RS20420 (LUX40_20420) 4231679..4234825 + 3147 WP_094955339.1 multidrug efflux RND transporter permease subunit AcrB -
LUX40_RS20425 (LUX40_20425) 4235308..4235682 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
LUX40_RS20430 (LUX40_20430) 4235709..4235927 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LUX40_RS20435 (LUX40_20435) 4236086..4236652 + 567 WP_016160393.1 maltose O-acetyltransferase -
LUX40_RS20440 (LUX40_20440) 4236624..4236752 - 129 Protein_4019 hypothetical protein -
LUX40_RS20445 (LUX40_20445) 4236789..4237259 + 471 WP_008805434.1 YlaC family protein -
LUX40_RS20450 (LUX40_20450) 4237228..4238685 - 1458 WP_032734891.1 PLP-dependent aminotransferase family protein -
LUX40_RS20455 (LUX40_20455) 4238786..4239484 + 699 WP_023297138.1 GNAT family protein -
LUX40_RS20460 (LUX40_20460) 4239481..4239621 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LUX40_RS20465 (LUX40_20465) 4239621..4239884 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T228146 WP_002892050.1 NZ_CP089385:4235709-4235927 [Klebsiella variicola subsp. variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T228146 NZ_CP123365:c1581986-1581699 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT228146 WP_008805436.1 NZ_CP089385:4235308-4235682 [Klebsiella variicola subsp. variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228146 NZ_CP123365:c1582225-1581986 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References