Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2787268..2788856 Replicon chromosome
Accession NZ_CP089385
Organism Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag LUX40_RS13525 Protein ID WP_008804919.1
Coordinates 2787268..2788590 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag LUX40_RS13530 Protein ID WP_008804920.1
Coordinates 2788590..2788856 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX40_RS13510 (LUX40_13510) 2782333..2784834 + 2502 WP_228665981.1 fimbria/pilus outer membrane usher protein -
LUX40_RS13515 (LUX40_13515) 2784822..2785826 + 1005 WP_063105598.1 fimbrial protein -
LUX40_RS13520 (LUX40_13520) 2785959..2787251 + 1293 WP_086070466.1 glycoside hydrolase family 10 protein -
LUX40_RS13525 (LUX40_13525) 2787268..2788590 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
LUX40_RS13530 (LUX40_13530) 2788590..2788856 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LUX40_RS13535 (LUX40_13535) 2789122..2789448 - 327 WP_008804921.1 hydroxyisourate hydrolase -
LUX40_RS13540 (LUX40_13540) 2789445..2789945 - 501 WP_023297604.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LUX40_RS13545 (LUX40_13545) 2789923..2791302 - 1380 WP_049155219.1 nucleobase:cation symporter-2 family protein -
LUX40_RS13550 (LUX40_13550) 2791648..2792802 + 1155 WP_008804924.1 FAD-dependent urate hydroxylase HpxO -
LUX40_RS13555 (LUX40_13555) 2792783..2793706 - 924 WP_008804925.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T228144 WP_008804919.1 NZ_CP089385:c2788590-2787268 [Klebsiella variicola subsp. variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T228144 NZ_CP123365:c1266156-1266049 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTGCCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT228144 WP_008804920.1 NZ_CP089385:c2788856-2788590 [Klebsiella variicola subsp. variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT228144 NZ_CP123365:1266204-1266270 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References