Detailed information of TA system TA228135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1885266..1885856
• Replicon: chromosome
• Accession: NZ_CP089385
• Organism: Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LUX40_RS09030
• Protein ID: WP_039104087.1
• Coordinates: 1885524..1885856 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: LUX40_RS09025
• Protein ID: WP_012541132.1
• Coordinates: 1885266..1885523 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX40_RS09005 (LUX40_09005)	1880874..1881449	+	576	WP_154045528.1	hypothetical protein	-
LUX40_RS09010 (LUX40_09010)	1881626..1882462	+	837	WP_008804155.1	alpha/beta hydrolase	-
LUX40_RS09015 (LUX40_09015)	1882669..1883640	+	972	WP_016161433.1	sensor domain-containing diguanylate cyclase	-
LUX40_RS09020 (LUX40_09020)	1883637..1884737	-	1101	WP_177334483.1	AarF/UbiB family protein	-
LUX40_RS09025 (LUX40_09025)	1885266..1885523	+	258	WP_012541132.1	antitoxin	Antitoxin
LUX40_RS09030 (LUX40_09030)	1885524..1885856	+	333	WP_039104087.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LUX40_RS09040 (LUX40_09040)	1886178..1887614	+	1437	WP_016161429.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LUX40_RS09050 (LUX40_09050)	1887987..1889441	-	1455	WP_008804170.1	AMP nucleosidase	-
LUX40_RS09055 (LUX40_09055)	1889572..1889817	-	246	WP_008804171.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11895.77 Da
• Isoelectric Point: 10.1839

>T228135 WP_039104087.1 NZ_CP089385:1885524-1885856 [Klebsiella variicola subsp. variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAIINEVLARLDAILS

Nucleotide

• Length: 333 bp

>T228135 NZ_CP123351:442363-442653 [Agrobacterium pusense]
ATGGGATTTCGCCTTTCTCTGCCTGCGGAAGAAGACGCCATCCGCATAGCGCAAGAAGGCATCGACCTATTTGGACCTCA
GCAAGCGCGAAAGTACCATCGCGAACTTTTCGCAATATTCGACTTGATATCACGGAATCCGAGGATCGCACGCGAGCGTA
ACGAGTTGTCGCCGTCGGTGCGCATTCATCCGTTCAGAGCACATCTGGTGGTCTATCGCGTTGAGGCGGACGACGACGTC
CTCATCGTCCGCGTCCGCCATGGGCACGAGGACTGGGGCAATGAGCCTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT228135 WP_012541132.1 NZ_CP089385:1885266-1885523 [Klebsiella variicola subsp. variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT228135 NZ_CP123351:442109-442360 [Agrobacterium pusense]
ATGGTGACCATGAATATTTCGCTGCCCGGCCAGATGAAGGAATGGGTCGAAGCGCAGTCTAAAACGGGCCGTTACTCCAA
TGCCAGCGACTATGTGCGAGATCTCATCCGAAAGGACCAGGAGCGGAGCGAAAAGATCGCTGCCATGCAGGGCCTAGTGG
ACGAAGGATTGCGGGCTCCCTCCGGTCATCGTTCTGAAGATGCCTTGTTTGCTGAGGCCGAAGCCCGCTCCATGAAACGT
AAAATTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3