Detailed information of TA system TA228134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1698469..1699466
• Replicon: chromosome
• Accession: NZ_CP089385
• Organism: Klebsiella variicola subsp. variicola strain KPN692

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LUX40_RS08240
• Protein ID: WP_229531768.1
• Coordinates: 1698912..1699466 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MDT5
• Locus tag: LUX40_RS08235
• Protein ID: WP_012541033.1
• Coordinates: 1698469..1698915 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX40_RS08220 (LUX40_08220)	1694764..1695762	+	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
LUX40_RS08225 (LUX40_08225)	1695881..1697401	+	1521	WP_232393189.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
LUX40_RS08230 (LUX40_08230)	1697417..1698427	+	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
LUX40_RS08235 (LUX40_08235)	1698469..1698915	-	447	WP_012541033.1	helix-turn-helix transcriptional regulator	Antitoxin
LUX40_RS08240 (LUX40_08240)	1698912..1699466	-	555	WP_229531768.1	HipA domain-containing protein	Toxin
LUX40_RS08245 (LUX40_08245)	1699495..1700016	-	522	WP_022064813.1	hypothetical protein	-
LUX40_RS08250 (LUX40_08250)	1700131..1700862	-	732	WP_008804033.1	outer membrane permeability protein SanA	-
LUX40_RS08255 (LUX40_08255)	1701155..1702039	-	885	WP_012541034.1	cytidine deaminase	-
LUX40_RS08260 (LUX40_08260)	1702168..1702863	-	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
LUX40_RS08265 (LUX40_08265)	1702853..1703257	-	405	WP_004201620.1	CidA/LrgA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (2-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20710.03 Da
• Isoelectric Point: 5.7891

>T228134 WP_229531768.1 NZ_CP089385:c1699466-1698912 [Klebsiella variicola subsp. variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 555 bp

>T228134 NZ_CP123351:c323051-322794 [Agrobacterium pusense]
TTGATCTGGAAAATTGAGTTTCAGCACCGCGCCGTCAAGGAGCTCGAAAAGCTGGCGAAGCAAGATGCGAACCGCATCGT
TTCGTTTCTGGCAGACAGGGTGGTGCAAAATGAAAATCCCCGCCAGACTGGTGCCGCCCTGCAAGGCTCCGAACTCGGCA
ATTATTGGCGCTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGATCATAAGCTCGTCGTGCTGGTGGTCGAA
ATCGGCCATCGCCGCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16396.05 Da
• Isoelectric Point: 10.5417

>AT228134 WP_012541033.1 NZ_CP089385:c1698915-1698469 [Klebsiella variicola subsp. variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT228134 NZ_CP123351:c323268-323035 [Agrobacterium pusense]
ATGAGCAAACAGACGGCCATCCGCCTCCCGGATGAGACCTATGAGCGGCTGAAAGCGCTTTCCGAGCGCACCGGACGCAC
ATCTGCCTATTACATTCGCGAGGCGATCGAGAAGCATATCGAAGATATGGAAGATCTTTATCTCGCCGAAGAGGCGACGC
GGCGACGGATCGCAAACAATGAGCGCACCTATACTCTGGAAGAAGTGATGCGCAATCTTGATCTGGAAAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MDT5