Detailed information of TA system TA022813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 69815..70391
• Replicon: plasmid pBIND01
• Accession: NC_010580
• Organism: Beijerinckia indica subsp. indica ATCC 9039

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BIND_RS19075
• Protein ID: WP_081433840.1
• Coordinates: 70140..70391 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B2ILA5
• Locus tag: BIND_RS19070
• Protein ID: WP_012382918.1
• Coordinates: 69815..70114 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIND_RS19045	66033..66743	-	711	WP_012382916.1	hypothetical protein	-
BIND_RS19050	66740..67384	-	645	WP_012382917.1	ParA family protein	-
BIND_RS20950	67775..68208	+	434	Protein_72	DUF1016 family protein	-
BIND_RS19060	68433..68654	-	222	Protein_73	aldo/keto reductase	-
BIND_RS19065	68746..69681	+	936	Protein_74	IS630 family transposase	-
BIND_RS19070	69815..70114	+	300	WP_012382918.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BIND_RS19075	70140..70391	+	252	WP_081433840.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BIND_RS20960	71052..71810	-	759	Protein_77	IS5 family transposase	-
BIND_RS19085	71869..73326	-	1458	WP_012382921.1	hypothetical protein	-
BIND_RS19090	73316..74650	-	1335	WP_012382922.1	DUF4365 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181736	181736
	-	flank	IS/Tn	-	-	69289..69681	392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-77)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-57)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9516.86 Da
• Isoelectric Point: 4.7824

>T22813 WP_081433840.1 NC_010580:70140-70391 [Beijerinckia indica subsp. indica ATCC 9039]
MLAIVDYISDDNPDAAQRLKDDIETKVSKLPDYPKLYREGRVIGTREMVVRSNYVIVYTEDARAVTILRVLHAAQQWPPI
GEE

Nucleotide

• Length: 252 bp

>T22813 NC_010580:70140-70391 [Beijerinckia indica subsp. indica ATCC 9039]
CTGCTGGCGATTGTGGACTACATATCGGACGATAATCCAGACGCGGCCCAGCGGCTCAAGGATGACATCGAGACCAAGGT
CTCGAAGTTGCCTGACTATCCAAAGCTCTACCGGGAGGGCCGTGTCATCGGTACGCGAGAAATGGTCGTGCGCTCGAATT
ACGTGATTGTGTATACAGAGGATGCTCGCGCTGTGACGATCTTGCGTGTTCTTCATGCGGCTCAGCAGTGGCCGCCCATA
GGGGAAGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11077.76 Da
• Isoelectric Point: 8.6980

>AT22813 WP_012382918.1 NC_010580:69815-70114 [Beijerinckia indica subsp. indica ATCC 9039]
MATQTSMLHIRVDDQLKAQATEKLANVGLTVSDAVRILLTRIAKEGGLPAGLTTEPEAYDAWFRAKVHEAIADPRPAIPH
QQVMDEMQFLIDRKRRARS

Nucleotide

• Length: 300 bp

>AT22813 NC_010580:69815-70114 [Beijerinckia indica subsp. indica ATCC 9039]
ATGGCCACTCAAACCTCGATGCTGCATATCCGTGTCGATGACCAACTGAAAGCACAGGCAACTGAGAAATTGGCCAACGT
CGGTCTGACCGTTTCTGATGCTGTGCGCATCCTGCTAACCCGGATCGCCAAGGAAGGTGGCTTGCCAGCCGGCCTGACGA
CTGAACCGGAAGCCTATGACGCCTGGTTCCGTGCCAAGGTTCACGAGGCGATCGCCGACCCTCGTCCAGCCATCCCGCAC
CAGCAGGTCATGGATGAGATGCAATTCCTGATCGATAGGAAGCGCCGTGCCCGTTCTTGA

Structures

Antitoxin

• AlphaFold DB: B2ILA5