Detailed information of TA system TA022812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 8344..8869
• Replicon: plasmid pBIND01
• Accession: NC_010580
• Organism: Beijerinckia indica subsp. indica ATCC 9039

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: B2IL40
• Locus tag: BIND_RS18760
• Protein ID: WP_012382853.1
• Coordinates: 8582..8869 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B2IL39
• Locus tag: BIND_RS18755
• Protein ID: WP_012382852.1
• Coordinates: 8344..8592 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BIND_RS18730	3364..3876	+	513	WP_012382847.1	lytic transglycosylase domain-containing protein	-
BIND_RS18735	3879..5024	+	1146	WP_210160547.1	hypothetical protein	-
BIND_RS21490	5276..5578	+	303	WP_148210824.1	hypothetical protein	-
BIND_RS18745	5588..5854	+	267	WP_012382850.1	hypothetical protein	-
BIND_RS18750	5886..8246	+	2361	WP_012382851.1	type IV secretion system DNA-binding domain-containing protein	-
BIND_RS18755	8344..8592	+	249	WP_012382852.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BIND_RS18760	8582..8869	+	288	WP_012382853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BIND_RS18765	9808..10167	+	360	WP_012382854.1	hypothetical protein	-
BIND_RS18770	10218..11063	-	846	WP_012382855.1	LysR family transcriptional regulator	-
BIND_RS18775	11154..11570	+	417	WP_012382856.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181736	181736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11215.98 Da
• Isoelectric Point: 10.2247

>T22812 WP_012382853.1 NC_010580:8582-8869 [Beijerinckia indica subsp. indica ATCC 9039]
MTYELAFVDAALKEWHKLDRNTREQFKKKLTERCENPRVPSAKLSGSTDRYKIKLRSVGYRLVYEVREAEILVVVIAIGK
RERSEVYKAAERRDL

Nucleotide

• Length: 288 bp

>T22812 NC_010580:8582-8869 [Beijerinckia indica subsp. indica ATCC 9039]
ATGACCTATGAGCTCGCTTTCGTGGATGCCGCTCTGAAAGAATGGCACAAGCTCGATAGGAACACGCGTGAGCAGTTCAA
GAAGAAGCTCACGGAACGCTGTGAAAATCCGCGCGTGCCCTCCGCAAAGCTCTCGGGAAGCACGGACCGGTACAAGATCA
AATTGCGCAGCGTTGGCTATCGGCTCGTCTATGAGGTTCGGGAAGCGGAAATTCTGGTGGTCGTGATTGCGATTGGGAAG
AGGGAACGCAGTGAAGTCTATAAAGCGGCCGAGCGACGAGACCTTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8841.16 Da
• Isoelectric Point: 4.2550

>AT22812 WP_012382852.1 NC_010580:8344-8592 [Beijerinckia indica subsp. indica ATCC 9039]
MAHIVLAEITASISELKKNPMGTVAAGEGAAVAILNRNEPAFYCVPARTYEAMLERLEDMELNAIADARANDPVIKVNLD
DL

Nucleotide

• Length: 249 bp

>AT22812 NC_010580:8344-8592 [Beijerinckia indica subsp. indica ATCC 9039]
ATGGCGCATATTGTTCTCGCTGAGATAACAGCCAGCATCTCGGAACTGAAAAAGAACCCCATGGGGACAGTAGCGGCTGG
AGAAGGCGCAGCAGTTGCGATCCTCAATCGCAACGAGCCAGCGTTTTATTGCGTGCCTGCCCGCACCTATGAGGCGATGC
TGGAACGTCTTGAAGATATGGAACTGAACGCCATTGCTGACGCGAGAGCGAATGATCCTGTCATCAAGGTCAATCTGGAT
GACCTATGA

Structures

Toxin

• AlphaFold DB: B2IL40

Antitoxin

• AlphaFold DB: B2IL39