Detailed information of TA system TA228111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 323736..324322
• Replicon: chromosome
• Accession: NZ_CP089383
• Organism: Klebsiella pneumoniae strain KPN342

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LUX38_RS01505
• Protein ID: WP_002920800.1
• Coordinates: 323954..324322 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LUX38_RS01500
• Protein ID: WP_004174006.1
• Coordinates: 323736..323957 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX38_RS01480 (319893)	319893..320819	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LUX38_RS01485 (320816)	320816..322093	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LUX38_RS01490 (322090)	322090..322857	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LUX38_RS01495 (322859)	322859..323572	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LUX38_RS01500 (323736)	323736..323957	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LUX38_RS01505 (323954)	323954..324322	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LUX38_RS01510 (324595)	324595..325911	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LUX38_RS01515 (326018)	326018..326905	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LUX38_RS01520 (326902)	326902..327747	+	846	WP_004185988.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LUX38_RS01525 (327749)	327749..328819	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	283728..329556	45828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T228111 WP_002920800.1 NZ_CP089383:323954-324322 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T228111 NZ_CP123282:c4518062-4517751 [Salmonella sp. SA14318]
ATGCAATTTATAGAAACGGAACTTTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT228111 WP_004174006.1 NZ_CP089383:323736-323957 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT228111 NZ_CP123282:c4517750-4517460 [Salmonella sp. SA14318]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCCATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCTGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7