Detailed information of TA system TA228105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 22003..22646
• Replicon: plasmid pKP062-1
• Accession: NZ_CP089326
• Organism: Klebsiella pneumoniae strain KP062

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LUX41_RS25890
• Protein ID: WP_016946796.1
• Coordinates: 22003..22419 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LUX41_RS25895
• Protein ID: WP_001261276.1
• Coordinates: 22416..22646 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX41_RS25880 (LUX41_25845)	18109..19206	+	1098	WP_049015454.1	RepB family plasmid replication initiator protein	-
LUX41_RS25885 (LUX41_25850)	20438..21557	+	1120	WP_088941746.1	IS3 family transposase	-
LUX41_RS25890 (LUX41_25855)	22003..22419	-	417	WP_016946796.1	type II toxin-antitoxin system VapC family toxin	Toxin
LUX41_RS25895 (LUX41_25860)	22416..22646	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LUX41_RS25900 (LUX41_25865)	22965..24125	+	1161	Protein_24	IS30 family transposase	-
LUX41_RS25905 (LUX41_25870)	24445..26640	-	2196	WP_032435683.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..172904	172904
	-	inside	IScluster/Tn	-	-	1..24125	24124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.56 Da
• Isoelectric Point: 8.5403

>T228105 WP_016946796.1 NZ_CP089326:c22419-22003 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T228105 NZ_CP123282:2361497-2361781 [Salmonella sp. SA14318]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT228105 WP_001261276.1 NZ_CP089326:c22646-22416 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT228105 NZ_CP123282:2361259-2361507 [Salmonella sp. SA14318]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023