Detailed information of TA system TA228103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5202308..5202933
• Replicon: chromosome
• Accession: NZ_CP089325
• Organism: Klebsiella pneumoniae strain KP062

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LUX41_RS25415
• Protein ID: WP_100105304.1
• Coordinates: 5202308..5202691 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: LUX41_RS25420
• Protein ID: WP_004150355.1
• Coordinates: 5202691..5202933 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX41_RS25400 (5199674)	5199674..5200576	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
LUX41_RS25405 (5200573)	5200573..5201208	+	636	WP_032430299.1	formate dehydrogenase cytochrome b556 subunit	-
LUX41_RS25410 (5201205)	5201205..5202134	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
LUX41_RS25415 (5202308)	5202308..5202691	-	384	WP_100105304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LUX41_RS25420 (5202691)	5202691..5202933	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
LUX41_RS25425 (5203138)	5203138..5204055	+	918	WP_032430300.1	alpha/beta hydrolase	-
LUX41_RS25430 (5204070)	5204070..5205011	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
LUX41_RS25435 (5205056)	5205056..5205493	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
LUX41_RS25440 (5205490)	5205490..5206350	-	861	WP_004146232.1	virulence factor BrkB family protein	-
LUX41_RS25445 (5206344)	5206344..5206943	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.60 Da
• Isoelectric Point: 6.8806

>T228103 WP_100105304.1 NZ_CP089325:c5202691-5202308 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIIMATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T228103 NZ_CP123282:c1023891-1023364 [Salmonella sp. SA14318]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT228103 WP_004150355.1 NZ_CP089325:c5202933-5202691 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT228103 NZ_CP123282:c1024178-1023888 [Salmonella sp. SA14318]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9