Detailed information of TA system TA228097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2591190..2592778
• Replicon: chromosome
• Accession: NZ_CP089325
• Organism: Klebsiella pneumoniae strain KP062

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: LUX41_RS12850
• Protein ID: WP_004176197.1
• Coordinates: 2591190..2592512 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LUX41_RS12855
• Protein ID: WP_032417111.1
• Coordinates: 2592512..2592778 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX41_RS12835 (2586246)	2586246..2588747	+	2502	WP_016831617.1	fimbria/pilus outer membrane usher protein	-
LUX41_RS12840 (2588735)	2588735..2589739	+	1005	WP_004176196.1	fimbrial protein	-
LUX41_RS12845 (2589881)	2589881..2591173	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LUX41_RS12850 (2591190)	2591190..2592512	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
LUX41_RS12855 (2592512)	2592512..2592778	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LUX41_RS12860 (2593043)	2593043..2593369	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LUX41_RS12865 (2593366)	2593366..2593866	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LUX41_RS12870 (2593844)	2593844..2595223	-	1380	WP_021440115.1	nucleobase:cation symporter-2 family protein	-
LUX41_RS12875 (2595569)	2595569..2596723	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LUX41_RS12880 (2596704)	2596704..2597627	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2401374..2625875	224501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T228097 WP_004176197.1 NZ_CP089325:c2592512-2591190 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T228097 NZ_CP123280:4077437-4077835 [Salmonella sp. SA17155]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT228097 WP_032417111.1 NZ_CP089325:c2592778-2592512 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT228097 NZ_CP123280:4077210-4077437 [Salmonella sp. SA17155]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A6T928