Detailed information of TA system TA022809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1389058..1389663
• Replicon: chromosome
• Accession: NC_010577
• Organism: Xylella fastidiosa M23

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: XFASM23_RS06250
• Protein ID: WP_004089252.1
• Coordinates: 1389058..1389354 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: XFASM23_RS06255
• Protein ID: WP_004089254.1
• Coordinates: 1389358..1389663 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFASM23_RS06225	1384223..1385200	+	978	WP_004087353.1	hypothetical protein	-
XFASM23_RS06230	1385459..1385905	+	447	WP_004087355.1	hypothetical protein	-
XFASM23_RS06235	1386009..1387253	-	1245	WP_011097983.1	phage tail protein	-
XFASM23_RS06240	1387257..1387817	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
XFASM23_RS06245	1387814..1388959	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
XFASM23_RS06250	1389058..1389354	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFASM23_RS06255	1389358..1389663	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
XFASM23_RS06260	1389674..1390027	-	354	WP_011097986.1	hypothetical protein	-
XFASM23_RS06265	1390024..1390665	-	642	WP_004090764.1	hypothetical protein	-
XFASM23_RS06270	1390662..1391492	-	831	WP_004090766.1	hypothetical protein	-
XFASM23_RS06275	1391489..1391806	-	318	WP_011097873.1	hypothetical protein	-
XFASM23_RS06280	1391806..1392576	-	771	WP_004090769.1	hypothetical protein	-
XFASM23_RS06285	1392624..1392914	+	291	WP_004087089.1	hypothetical protein	-
XFASM23_RS06290	1392898..1393164	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1386009..1404308	18299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T22809 WP_004089252.1 NC_010577:1389058-1389354 [Xylella fastidiosa M23]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T22809 NC_010577:1389058-1389354 [Xylella fastidiosa M23]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT22809 WP_004089254.1 NC_010577:1389358-1389663 [Xylella fastidiosa M23]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT22809 NC_010577:1389358-1389663 [Xylella fastidiosa M23]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9