228084

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 46656..47263 Replicon plasmid p2
Accession NZ_CP089321
Organism Enterococcus faecium strain NMVRE-001

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag LUB18_RS15240 Protein ID WP_002321032.1
Coordinates 46913..47263 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag LUB18_RS15235 Protein ID WP_002287514.1
Coordinates 46656..46919 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUB18_RS15195 (LUB18_15195) 42006..42479 + 474 WP_002321302.1 single-stranded DNA-binding protein -
LUB18_RS15200 (LUB18_15200) 42845..43105 + 261 WP_002321303.1 hypothetical protein -
LUB18_RS15205 (LUB18_15205) 43550..43756 + 207 WP_002296239.1 hypothetical protein -
LUB18_RS15210 (LUB18_15210) 43756..44007 + 252 WP_002305766.1 hypothetical protein -
LUB18_RS15215 (LUB18_15215) 44023..44439 + 417 WP_002305764.1 hypothetical protein -
LUB18_RS15220 (LUB18_15220) 44421..44687 + 267 Protein_44 hypothetical protein -
LUB18_RS15225 (LUB18_15225) 44860..45261 + 402 WP_002305759.1 IS200/IS605 family transposase -
LUB18_RS15230 (LUB18_15230) 45273..46418 + 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
LUB18_RS15235 (LUB18_15235) 46656..46919 + 264 WP_002287514.1 hypothetical protein Antitoxin
LUB18_RS15240 (LUB18_15240) 46913..47263 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LUB18_RS15245 (LUB18_15245) 47361..48662 - 1302 WP_002302440.1 ISL3 family transposase -
LUB18_RS15250 (LUB18_15250) 48788..49402 - 615 WP_002318230.1 recombinase family protein -
LUB18_RS15255 (LUB18_15255) 49852..51177 + 1326 WP_002348911.1 Y-family DNA polymerase -
LUB18_RS15260 (LUB18_15260) 51170..51520 + 351 WP_002303114.1 hypothetical protein -
LUB18_RS15265 (LUB18_15265) 51517..51696 + 180 WP_002322386.1 hypothetical protein -
LUB18_RS15270 (LUB18_15270) 51708..52001 + 294 WP_002322251.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..59066 59066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T228084 WP_002321032.1 NZ_CP089321:46913-47263 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T228084 NZ_CP123280:345140-345295 [Salmonella sp. SA17155]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCGCTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT228084 WP_002287514.1 NZ_CP089321:46656-46919 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT228084 NZ_CP123280:c345128-345070 [Salmonella sp. SA17155]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References