Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 497902..498473 Replicon chromosome
Accession NZ_CP089319
Organism Enterococcus faecium strain NMVRE-001

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LUB18_RS02360 Protein ID WP_002286801.1
Coordinates 498132..498473 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LUB18_RS02355 Protein ID WP_002323011.1
Coordinates 497902..498132 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUB18_RS02330 (LUB18_02330) 493035..494366 + 1332 WP_232407653.1 FAD-containing oxidoreductase -
LUB18_RS02335 (LUB18_02335) 494388..495014 + 627 WP_002286816.1 cysteine hydrolase -
LUB18_RS02340 (LUB18_02340) 495197..495778 + 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
LUB18_RS02345 (LUB18_02345) 496510..497072 + 563 Protein_467 SOS response-associated peptidase family protein -
LUB18_RS02350 (LUB18_02350) 497277..497615 - 339 WP_002286804.1 hypothetical protein -
LUB18_RS02355 (LUB18_02355) 497902..498132 + 231 WP_002323011.1 hypothetical protein Antitoxin
LUB18_RS02360 (LUB18_02360) 498132..498473 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LUB18_RS02365 (LUB18_02365) 499323..499508 + 186 WP_002304207.1 hypothetical protein -
LUB18_RS02370 (LUB18_02370) 500013..500207 + 195 WP_002297028.1 hypothetical protein -
LUB18_RS02375 (LUB18_02375) 500398..500647 + 250 Protein_473 transposase -
LUB18_RS02380 (LUB18_02380) 500891..501721 - 831 WP_002286789.1 manganese catalase family protein -
LUB18_RS02385 (LUB18_02385) 501929..503263 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T228083 WP_002286801.1 NZ_CP089319:498132-498473 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T228083 NZ_CP123280:c332748-332554 [Salmonella sp. SA17155]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACAG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT228083 WP_002323011.1 NZ_CP089319:497902-498132 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT228083 NZ_CP123280:332379-332555 [Salmonella sp. SA17155]
GGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGAGA
GCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTTCA
ATAGTGGCAGTTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References