Detailed information of TA system TA228078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3315433..3316454
• Replicon: chromosome
• Accession: NZ_CP089317
• Organism: Proteus mirabilis strain PM1162

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LUA10_RS15935
• Protein ID: WP_004249488.1
• Coordinates: 3315885..3316454 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: LUA10_RS15930
• Protein ID: WP_004245528.1
• Coordinates: 3315433..3315885 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUA10_RS15910 (LUA10_15910)	3311219..3311749	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
LUA10_RS15915 (LUA10_15915)	3312066..3313031	+	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
LUA10_RS15920 (LUA10_15920)	3313031..3314611	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
LUA10_RS15925 (LUA10_15925)	3314604..3315305	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
LUA10_RS15930 (LUA10_15930)	3315433..3315885	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
LUA10_RS15935 (LUA10_15935)	3315885..3316454	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
LUA10_RS15940 (LUA10_15940)	3316570..3318924	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T228078 WP_004249488.1 NZ_CP089317:3315885-3316454 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T228078 NZ_CP123267:45282-45698 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT228078 WP_004245528.1 NZ_CP089317:3315433-3315885 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT228078 NZ_CP123267:45055-45285 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3