Detailed information of TA system TA228077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1802823..1803622
• Replicon: chromosome
• Accession: NZ_CP089317
• Organism: Proteus mirabilis strain PM1162

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: LUA10_RS08765
• Protein ID: WP_004247966.1
• Coordinates: 1803098..1803622 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: LUA10_RS08760
• Protein ID: WP_004247964.1
• Coordinates: 1802823..1803101 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUA10_RS08735 (LUA10_08735)	1798173..1799255	+	1083	WP_004242680.1	peptide chain release factor 1	-
LUA10_RS08740 (LUA10_08740)	1799255..1800103	+	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
LUA10_RS08745 (LUA10_08745)	1800081..1800896	+	816	WP_004242688.1	invasion regulator SirB1	-
LUA10_RS08750 (LUA10_08750)	1800954..1801808	+	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
LUA10_RS08755 (LUA10_08755)	1801816..1802745	+	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
LUA10_RS08760 (LUA10_08760)	1802823..1803101	+	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
LUA10_RS08765 (LUA10_08765)	1803098..1803622	+	525	WP_004247966.1	hypothetical protein	Toxin
LUA10_RS08770 (LUA10_08770)	1803703..1805403	-	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
LUA10_RS08780 (LUA10_08780)	1806367..1807308	+	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T228077 WP_004247966.1 NZ_CP089317:1803098-1803622 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T228077 NZ_CP123266:c4699279-4698968 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT228077 WP_004247964.1 NZ_CP089317:1802823-1803101 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT228077 NZ_CP123266:c4698967-4698677 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: B4EVS2