Detailed information of TA system TA228076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1523474..1524119
• Replicon: chromosome
• Accession: NZ_CP089317
• Organism: Proteus mirabilis strain PM1162

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B4ET86
• Locus tag: LUA10_RS07285
• Protein ID: WP_004247035.1
• Coordinates: 1523934..1524119 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B4ET85
• Locus tag: LUA10_RS07280
• Protein ID: WP_004247034.1
• Coordinates: 1523474..1523887 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUA10_RS07250 (LUA10_07250)	1519027..1519185	-	159	WP_004247025.1	hypothetical protein	-
LUA10_RS07255 (LUA10_07255)	1519637..1519813	+	177	WP_004247793.1	hypothetical protein	-
LUA10_RS07260 (LUA10_07260)	1519984..1520730	-	747	Protein_1396	methyltransferase domain-containing protein	-
LUA10_RS07265 (LUA10_07265)	1521580..1522158	+	579	WP_017628400.1	alpha/beta hydrolase	-
LUA10_RS07270 (LUA10_07270)	1522271..1522513	-	243	WP_230458803.1	hypothetical protein	-
LUA10_RS07275 (LUA10_07275)	1522878..1523378	-	501	WP_004247032.1	response regulator	-
LUA10_RS07280 (LUA10_07280)	1523474..1523887	-	414	WP_004247034.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LUA10_RS07285 (LUA10_07285)	1523934..1524119	-	186	WP_004247035.1	type II toxin-antitoxin system HicA family toxin	Toxin
LUA10_RS07290 (LUA10_07290)	1524225..1524692	+	468	WP_223293438.1	DUF1073 domain-containing protein	-
LUA10_RS07295 (LUA10_07295)	1524635..1525003	+	369	WP_223293455.1	DUF1073 domain-containing protein	-
LUA10_RS07300 (LUA10_07300)	1525009..1525230	+	222	Protein_1404	hypothetical protein	-
LUA10_RS07305 (LUA10_07305)	1525559..1526110	-	552	WP_017628396.1	PH domain-containing protein	-
LUA10_RS07310 (LUA10_07310)	1526174..1526488	-	315	WP_017628395.1	hypothetical protein	-
LUA10_RS07320 (LUA10_07320)	1526895..1528628	-	1734	WP_017628394.1	LPS biosynthesis-modulating metalloenzyme YejM	-
LUA10_RS07325 (LUA10_07325)	1528652..1528879	-	228	WP_004247042.1	YejL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1513599..1536101	22502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7049.30 Da
• Isoelectric Point: 10.9659

>T228076 WP_004247035.1 NZ_CP089317:c1524119-1523934 [Proteus mirabilis]
MKSSELIKLLEKNGWILDRIKGSHHQFTHPDFSFVVTVPHPRKDLKKGTLNQIIKSAKLKN

Nucleotide

• Length: 186 bp

>T228076 NZ_CP123266:c4422231-4422058 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15251.98 Da
• Isoelectric Point: 4.3830

>AT228076 WP_004247034.1 NZ_CP089317:c1523887-1523474 [Proteus mirabilis]
MLYPAFIEIDEDGSASGWFPDIDGCIFAGDSIEEAHADARSAIDAHFELLSEKGLDIPSPKSQQEHLINSSGDYNNGIWL
LVDVDMDKYDGRAERINITLPHRLLSRIDTIVKTNPEYGSRSGFIATATRKELQKNI

Nucleotide

• Length: 414 bp

>AT228076 NZ_CP123266:c4422555-4422259 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: B4ET86

Antitoxin

• AlphaFold DB: B4ET85