Detailed information of TA system TA228075

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 5026323..5026999
• Replicon: chromosome
• Accession: NZ_CP089316
• Organism: Citrobacter portucalensis strain K218

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LT989_RS24430
• Protein ID: WP_110249215.1
• Coordinates: 5026661..5026999 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: LT989_RS24425
• Protein ID: WP_126957115.1
• Coordinates: 5026323..5026625 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT989_RS24385 (5021585)	5021585..5022406	+	822	WP_110249207.1	DUF932 domain-containing protein	-
LT989_RS24390 (5022623)	5022623..5023324	+	702	WP_126957107.1	WYL domain-containing protein	-
LT989_RS24395 (5023507)	5023507..5024202	+	696	WP_126957109.1	hypothetical protein	-
LT989_RS24400 (5024224)	5024224..5024697	+	474	WP_110249210.1	hypothetical protein	-
LT989_RS24405 (5024776)	5024776..5025015	+	240	Protein_4767	DUF905 domain-containing protein	-
LT989_RS24410 (5025110)	5025110..5025568	+	459	WP_126957111.1	antirestriction protein	-
LT989_RS24415 (5025584)	5025584..5026060	+	477	WP_126957113.1	RadC family protein	-
LT989_RS24420 (5026069)	5026069..5026290	+	222	WP_110249213.1	DUF987 domain-containing protein	-
LT989_RS24425 (5026323)	5026323..5026625	+	303	WP_126957115.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LT989_RS24430 (5026661)	5026661..5026999	+	339	WP_110249215.1	TA system toxin CbtA family protein	Toxin
LT989_RS24435 (5027114)	5027114..5027947	+	834	WP_110249234.1	DUF4942 domain-containing protein	-
LT989_RS24440 (5028180)	5028180..5028977	+	798	WP_110249216.1	helix-turn-helix transcriptional regulator	-
LT989_RS24445 (5029137)	5029137..5030009	-	873	WP_126957117.1	HNH endonuclease	-
LT989_RS24450 (5030511)	5030511..5031767	-	1257	WP_126957119.1	FAD/NAD(P)-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5018156..5026999	8843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (2-99)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12794.80 Da
• Isoelectric Point: 10.1435

>T228075 WP_110249215.1 NZ_CP089316:5026661-5026999 [Citrobacter portucalensis]
MKSLPANQRVAKPCPPPVLVWQTLLTRLLKQHYGLALNDTPFSDENVIQEHINAGITLADAVNFLVEKYELVRIDRRGFN
CQKQSPYLRAVDILRARQATGLLRQSYHPSTR

Nucleotide

• Length: 339 bp

>T228075 NZ_CP123266:c4324615-4324265 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11497.99 Da
• Isoelectric Point: 5.5420

>AT228075 WP_126957115.1 NZ_CP089316:5026323-5026625 [Citrobacter portucalensis]
MGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTDELNPRHAHCITLYQDELTC
EADTLGSYGYVYIVIYPTQR

Nucleotide

• Length: 303 bp

>AT228075 NZ_CP123266:c4324860-4324609 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCTTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA