Detailed information of TA system TA228074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4543527..4544143
• Replicon: chromosome
• Accession: NZ_CP089316
• Organism: Citrobacter portucalensis strain K218

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LT989_RS22160
• Protein ID: WP_126957449.1
• Coordinates: 4543769..4544143 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: LT989_RS22155
• Protein ID: WP_003825279.1
• Coordinates: 4543527..4543769 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT989_RS22120 (4538934)	4538934..4539533	+	600	WP_003825293.1	glucose-1-phosphatase	-
LT989_RS22125 (4539527)	4539527..4540399	+	873	WP_008786998.1	virulence factor BrkB family protein	-
LT989_RS22130 (4540396)	4540396..4540833	+	438	WP_126957455.1	D-aminoacyl-tRNA deacylase	-
LT989_RS22135 (4540878)	4540878..4541820	+	943	Protein_4333	fatty acid biosynthesis protein FabY	-
LT989_RS22140 (4541826)	4541826..4542113	-	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LT989_RS22145 (4542106)	4542106..4542348	-	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
LT989_RS22150 (4542413)	4542413..4543321	-	909	WP_126957451.1	alpha/beta hydrolase	-
LT989_RS22155 (4543527)	4543527..4543769	+	243	WP_003825279.1	CopG family transcriptional regulator	Antitoxin
LT989_RS22160 (4543769)	4543769..4544143	+	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
LT989_RS22165 (4544180)	4544180..4545109	-	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
LT989_RS22170 (4545106)	4545106..4545741	-	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
LT989_RS22175 (4545738)	4545738..4546640	-	903	WP_003825273.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T228074 WP_126957449.1 NZ_CP089316:4543769-4544143 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T228074 NZ_CP123266:c4205591-4205214 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGTGGGCGAAACAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT228074 WP_003825279.1 NZ_CP089316:4543527-4543769 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT228074 NZ_CP123266:c4206049-4205681 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8