Detailed information of TA system TA228063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1744658..1745394
• Replicon: chromosome
• Accession: NZ_CP089316
• Organism: Citrobacter portucalensis strain K218

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A7W3F8J0
• Locus tag: LT989_RS08545
• Protein ID: WP_063941970.1
• Coordinates: 1744658..1745140 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LT989_RS08550
• Protein ID: WP_008784769.1
• Coordinates: 1745128..1745394 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT989_RS08510 (1739659)	1739659..1740573	+	915	WP_063941590.1	bestrophin family protein	-
LT989_RS08515 (1740570)	1740570..1740947	-	378	WP_003832953.1	GFA family protein	-
LT989_RS08520 (1741079)	1741079..1741837	-	759	WP_003832955.1	trans-aconitate 2-methyltransferase	-
LT989_RS08525 (1741948)	1741948..1742469	+	522	WP_003832958.1	GNAT family N-acetyltransferase	-
LT989_RS08530 (1742479)	1742479..1743315	-	837	WP_003832960.1	DMT family transporter	-
LT989_RS08535 (1743368)	1743368..1743745	-	378	WP_003832962.1	hypothetical protein	-
LT989_RS08540 (1744198)	1744198..1744404	+	207	Protein_1672	hypothetical protein	-
LT989_RS08545 (1744658)	1744658..1745140	-	483	WP_063941970.1	GNAT family N-acetyltransferase	Toxin
LT989_RS08550 (1745128)	1745128..1745394	-	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
LT989_RS08555 (1745548)	1745548..1747530	-	1983	WP_003832969.1	alkyl sulfatase dimerization domain-containing protein	-
LT989_RS08560 (1747786)	1747786..1748172	+	387	WP_063941972.1	hypothetical protein	-
LT989_RS08565 (1748289)	1748289..1748918	-	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
LT989_RS08570 (1749156)	1749156..1750343	-	1188	WP_063941973.1	outer membrane protein transport protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17613.41 Da
• Isoelectric Point: 9.5814

>T228063 WP_063941970.1 NZ_CP089316:c1745140-1744658 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALTDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T228063 NZ_CP123266:1038087-1038422 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT228063 WP_008784769.1 NZ_CP089316:c1745394-1745128 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT228063 NZ_CP123266:1037839-1038087 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3F8J0