Detailed information of TA system TA228062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1276598..1277188
• Replicon: chromosome
• Accession: NZ_CP089316
• Organism: Citrobacter portucalensis strain K218

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LT989_RS06110
• Protein ID: WP_061372072.1
• Coordinates: 1276856..1277188 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LT989_RS06105
• Protein ID: WP_126956015.1
• Coordinates: 1276598..1276855 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT989_RS06090 (1272891)	1272891..1274933	+	2043	WP_126956009.1	hypothetical protein	-
LT989_RS06095 (1275573)	1275573..1276046	+	474	WP_126956011.1	hypothetical protein	-
LT989_RS06100 (1276043)	1276043..1276249	+	207	WP_126956013.1	helix-turn-helix transcriptional regulator	-
LT989_RS06105 (1276598)	1276598..1276855	+	258	WP_126956015.1	antitoxin	Antitoxin
LT989_RS06110 (1276856)	1276856..1277188	+	333	WP_061372072.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LT989_RS06115 (1277257)	1277257..1277466	-	210	WP_126956017.1	hypothetical protein	-
LT989_RS06120 (1278036)	1278036..1278920	+	885	WP_126956019.1	integrase domain-containing protein	-
LT989_RS06125 (1279722)	1279722..1280984	-	1263	WP_126956021.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1264360..1277188	12828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11816.58 Da
• Isoelectric Point: 9.3417

>T228062 WP_061372072.1 NZ_CP089316:1276856-1277188 [Citrobacter portucalensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T228062 NZ_CP123266:901579-901986 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9378.90 Da
• Isoelectric Point: 4.4620

>AT228062 WP_126956015.1 NZ_CP089316:1276598-1276855 [Citrobacter portucalensis]
MYITRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT228062 NZ_CP123266:901332-901598 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA