Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 388907..389433 Replicon chromosome
Accession NZ_CP089316
Organism Citrobacter portucalensis strain K218

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LT989_RS01925 Protein ID WP_000323025.1
Coordinates 389146..389433 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag LT989_RS01920 Protein ID WP_004196370.1
Coordinates 388907..389146 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT989_RS01895 (384610) 384610..384927 - 318 WP_126957816.1 TA system toxin CbtA family protein -
LT989_RS01900 (384987) 384987..386036 + 1050 Protein_368 ISNCY family transposase -
LT989_RS01905 (386949) 386949..387521 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
LT989_RS01910 (387721) 387721..388644 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
LT989_RS01915 (388778) 388778..388882 - 105 Protein_371 protein YdfV -
LT989_RS01920 (388907) 388907..389146 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LT989_RS01925 (389146) 389146..389433 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LT989_RS01930 (389854) 389854..390090 + 237 WP_003830891.1 hypothetical protein -
LT989_RS01935 (390192) 390192..390821 - 630 WP_003830892.1 LysE family translocator -
LT989_RS01940 (390931) 390931..391836 + 906 WP_126955957.1 LysR substrate-binding domain-containing protein -
LT989_RS01945 (391961) 391961..392944 + 984 WP_126955956.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T228060 WP_000323025.1 NZ_CP089316:389146-389433 [Citrobacter portucalensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T228060 NZ_CP123266:c595010-594546 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT228060 WP_004196370.1 NZ_CP089316:388907-389146 [Citrobacter portucalensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT228060 NZ_CP123266:c595345-595010 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References