Detailed information of TA system TA228058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5336856..5337432
• Replicon: chromosome
• Accession: NZ_CP089315
• Organism: Klebsiella michiganensis strain K92

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: LT984_RS25445
• Protein ID: WP_042933975.1
• Coordinates: 5337145..5337432 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LT984_RS25440
• Protein ID: WP_014837314.1
• Coordinates: 5336856..5337158 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT984_RS25420 (LT984_25420)	5331942..5333345	+	1404	WP_089046410.1	restriction endonuclease subunit S	-
LT984_RS25425 (LT984_25425)	5333696..5334031	+	336	WP_014837316.1	endoribonuclease SymE	-
LT984_RS25430 (LT984_25430)	5334484..5335395	+	912	WP_014837315.1	acetamidase/formamidase family protein	-
LT984_RS25435 (LT984_25435)	5335392..5336735	+	1344	WP_004128506.1	APC family permease	-
LT984_RS25440 (LT984_25440)	5336856..5337158	-	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
LT984_RS25445 (LT984_25445)	5337145..5337432	-	288	WP_042933975.1	BrnT family toxin	Toxin
LT984_RS25450 (LT984_25450)	5337691..5338134	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
LT984_RS25455 (LT984_25455)	5338128..5339026	-	899	Protein_5009	LysR family transcriptional regulator	-
LT984_RS25460 (LT984_25460)	5339114..5339896	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
LT984_RS25465 (LT984_25465)	5340044..5340628	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
LT984_RS25470 (LT984_25470)	5340774..5341574	+	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
LT984_RS25475 (LT984_25475)	5341571..5342089	+	519	WP_014837307.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T228058 WP_042933975.1 NZ_CP089315:c5337432-5337145 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T228058 NZ_CP123266:268889-269119 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT228058 WP_014837314.1 NZ_CP089315:c5337158-5336856 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT228058 NZ_CP123266:269116-269475 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2