228056

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4639248..4639867 Replicon chromosome
Accession NZ_CP089315
Organism Klebsiella michiganensis strain K92

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LT984_RS22205 Protein ID WP_004099646.1
Coordinates 4639649..4639867 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag LT984_RS22200 Protein ID WP_004129911.1
Coordinates 4639248..4639622 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT984_RS22190 (LT984_22190) 4634406..4635599 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LT984_RS22195 (LT984_22195) 4635622..4638768 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LT984_RS22200 (LT984_22200) 4639248..4639622 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
LT984_RS22205 (LT984_22205) 4639649..4639867 + 219 WP_004099646.1 HHA domain-containing protein Toxin
LT984_RS22210 (LT984_22210) 4640030..4640596 + 567 WP_014837607.1 maltose O-acetyltransferase -
LT984_RS22215 (LT984_22215) 4640568..4640702 - 135 WP_223226764.1 hypothetical protein -
LT984_RS22220 (LT984_22220) 4640723..4641193 + 471 WP_014228205.1 YlaC family protein -
LT984_RS22225 (LT984_22225) 4641168..4642622 - 1455 WP_014837606.1 PLP-dependent aminotransferase family protein -
LT984_RS22230 (LT984_22230) 4642724..4643422 + 699 WP_014228203.1 GNAT family protein -
LT984_RS22235 (LT984_22235) 4643419..4643559 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LT984_RS22240 (LT984_22240) 4643559..4643822 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T228056 WP_004099646.1 NZ_CP089315:4639649-4639867 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T228056 NZ_CP123266:193740-193847 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT228056 WP_004129911.1 NZ_CP089315:4639248-4639622 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT228056 NZ_CP123266:c193683-193628 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References