Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 873419..874076 Replicon chromosome
Accession NZ_CP089315
Organism Klebsiella michiganensis strain K92

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag LT984_RS04230 Protein ID WP_004854060.1
Coordinates 873666..874076 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag LT984_RS04225 Protein ID WP_004124953.1
Coordinates 873419..873685 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT984_RS04210 (LT984_04210) 868653..869078 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
LT984_RS04215 (LT984_04215) 869199..871997 - 2799 WP_014839506.1 transcriptional regulator DagR -
LT984_RS04220 (LT984_04220) 872191..873174 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
LT984_RS04225 (LT984_04225) 873419..873685 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
LT984_RS04230 (LT984_04230) 873666..874076 + 411 WP_004854060.1 protein YgfX Toxin
LT984_RS04235 (LT984_04235) 874085..874606 - 522 WP_014226792.1 flavodoxin FldB -
LT984_RS04240 (LT984_04240) 874728..875624 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
LT984_RS04245 (LT984_04245) 875647..876360 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LT984_RS04250 (LT984_04250) 876366..878099 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T228047 WP_004854060.1 NZ_CP089315:873666-874076 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T228047 NZ_CP123260:c4392630-4392457 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT228047 WP_004124953.1 NZ_CP089315:873419-873685 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT228047 NZ_CP123260:c4392954-4392658 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References