Detailed information of TA system TA228046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 57050..57597
• Replicon: chromosome
• Accession: NZ_CP089315
• Organism: Klebsiella michiganensis strain K92

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LT984_RS00290
• Protein ID: WP_014839830.1
• Coordinates: 57050..57358 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3H0P7
• Locus tag: LT984_RS00295
• Protein ID: WP_014227298.1
• Coordinates: 57361..57597 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT984_RS00260 (LT984_00260)	52697..53008	-	312	WP_014839834.1	PTS sugar transporter subunit IIB	-
LT984_RS00265 (LT984_00265)	53303..54244	+	942	WP_014227303.1	LacI family DNA-binding transcriptional regulator	-
LT984_RS00270 (LT984_00270)	54268..54561	-	294	WP_014227302.1	YicS family protein	-
LT984_RS00275 (LT984_00275)	54771..55736	+	966	WP_014839832.1	Rpn family recombination-promoting nuclease/putative transposase	-
LT984_RS00280 (LT984_00280)	55811..55993	+	183	WP_014839831.1	hypothetical protein	-
LT984_RS00285 (LT984_00285)	55997..56899	-	903	WP_014227300.1	EamA family transporter	-
LT984_RS00290 (LT984_00290)	57050..57358	-	309	WP_014839830.1	CcdB family protein	Toxin
LT984_RS00295 (LT984_00295)	57361..57597	-	237	WP_014227298.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LT984_RS00300 (LT984_00300)	57703..59136	-	1434	WP_014839829.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
LT984_RS00305 (LT984_00305)	59161..60063	-	903	WP_009654080.1	N-acetylmuramic acid 6-phosphate etherase	-
LT984_RS00310 (LT984_00310)	60224..61390	-	1167	WP_014839828.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11724.63 Da
• Isoelectric Point: 8.8012

>T228046 WP_014839830.1 NZ_CP089315:c57358-57050 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGKE
VCNLNHQREVVKASMDFLFDGI

Nucleotide

• Length: 309 bp

>T228046 NZ_CP123260:c4296114-4295764 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8783.92 Da
• Isoelectric Point: 5.7928

>AT228046 WP_014227298.1 NZ_CP089315:c57597-57361 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT228046 NZ_CP123260:c4296359-4296108 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H0P7