Detailed information of TA system TA228026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 858875..859500
• Replicon: chromosome
• Accession: NZ_CP089313
• Organism: Salmonella enterica subsp. enterica serovar Indiana strain YZ21MCS4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LT987_RS04220
• Protein ID: WP_000911337.1
• Coordinates: 859102..859500 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C0PXM4
• Locus tag: LT987_RS04215
• Protein ID: WP_000557545.1
• Coordinates: 858875..859102 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT987_RS04185 (853920)	853920..855437	+	1518	WP_000003339.1	lysine--tRNA ligase	-
LT987_RS04190 (855513)	855513..856058	-	546	WP_000133994.1	isopentenyl-diphosphate Delta-isomerase	-
LT987_RS04195 (856323)	856323..857081	+	759	WP_000244318.1	amidase activator ActS	-
LT987_RS04205 (857366)	857366..858172	-	807	WP_010989071.1	DUF1460 domain-containing protein	-
LT987_RS04210 (858447)	858447..858707	-	261	WP_023227725.1	hypothetical protein	-
LT987_RS04215 (858875)	858875..859102	+	228	WP_000557545.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LT987_RS04220 (859102)	859102..859500	+	399	WP_000911337.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LT987_RS04225 (860304)	860304..860840	+	537	WP_023227726.1	STM3031 family outer membrane protein	-
LT987_RS04230 (860887)	860887..861519	+	633	WP_023227727.1	YfdX family protein	-
LT987_RS04235 (862238)	862238..862825	+	588	WP_023227728.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	857366..868693	11327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15037.43 Da
• Isoelectric Point: 7.7785

>T228026 WP_000911337.1 NZ_CP089313:859102-859500 [Salmonella enterica subsp. enterica serovar Indiana]
MLKFMLDTNTCIFTIKNKPEHIRERFNLNTSRMCISSITLMELIYGAEKSLAPERNLAVVEGFISRLEVLDYDTQAAIHT
GQIRAELARKGTPVGPYDQMIAGHARSRGLVVVTNNLREFERIPGIRIEDWC

Nucleotide

• Length: 399 bp

>T228026 NZ_CP123256:c55643-55263 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACATATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8500.48 Da
• Isoelectric Point: 5.0053

>AT228026 WP_000557545.1 NZ_CP089313:858875-859102 [Salmonella enterica subsp. enterica serovar Indiana]
MHTTLFFSNRTQAVRLPKSISFPDDVKHVEIIAVGRSRIITPVGESWDSWFDGEGASTDFMSTREQPAVQEREGF

Nucleotide

• Length: 228 bp

>AT228026 NZ_CP123256:c55864-55643 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0R9MGW6