Detailed information of TA system TA228021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5339232..5339866
• Replicon: chromosome
• Accession: NZ_CP089312
• Organism: Streptomyces sp. GQFP

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LUX31_RS23655
• Protein ID: WP_235476972.1
• Coordinates: 5339232..5339654 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A101UDL3
• Locus tag: LUX31_RS23660
• Protein ID: WP_053752751.1
• Coordinates: 5339651..5339866 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX31_RS23640 (LUX31_23640)	5334502..5335842	-	1341	WP_235476969.1	right-handed parallel beta-helix repeat-containing protein	-
LUX31_RS23645 (LUX31_23645)	5335930..5336289	-	360	WP_235476970.1	hypothetical protein	-
LUX31_RS23650 (LUX31_23650)	5336286..5338784	-	2499	WP_235476971.1	SpoIIE family protein phosphatase	-
LUX31_RS23655 (LUX31_23655)	5339232..5339654	-	423	WP_235476972.1	PIN domain nuclease	Toxin
LUX31_RS23660 (LUX31_23660)	5339651..5339866	-	216	WP_053752751.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LUX31_RS23665 (LUX31_23665)	5339985..5342414	-	2430	WP_235476973.1	helicase-associated domain-containing protein	-
LUX31_RS23670 (LUX31_23670)	5342675..5342868	+	194	Protein_4707	restriction endonuclease	-
LUX31_RS23675 (LUX31_23675)	5342849..5344285	-	1437	WP_235476974.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-46)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15402.32 Da
• Isoelectric Point: 4.7222

>T228021 WP_235476972.1 NZ_CP089312:c5339654-5339232 [Streptomyces sp. GQFP]
VNAAQFLIDTSALARFMREDAEQYGWDQAAAAGLIATCPITELEFFYSARSAADRACGIEDMRLIFGWVPVDDRAYDRAW
QVQEALTKQGKHRSAGAVDLVVAATAELQGLTLLHRDHDFVRIAAVTGQALQWYGPESGK

Nucleotide

• Length: 423 bp

>T228021 NZ_CP123255:c3820211-3819813 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7803.82 Da
• Isoelectric Point: 8.6595

>AT228021 WP_053752751.1 NZ_CP089312:c5339866-5339651 [Streptomyces sp. GQFP]
VSRTVIDLDDDLVADVAKALGTSTKKETVNTALREVLESRRRALALARLRAAAGDGAFDLELFENKGNYRR

Nucleotide

• Length: 216 bp

>AT228021 NZ_CP123255:c3820507-3820214 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A101UDL3