Detailed information of TA system TA228020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4699616..4700645
• Replicon: chromosome
• Accession: NZ_CP089312
• Organism: Streptomyces sp. GQFP

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LUX31_RS20730
• Protein ID: WP_235476296.1
• Coordinates: 4700157..4700645 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LUX31_RS20725
• Protein ID: WP_235476289.1
• Coordinates: 4699616..4700074 (+)
• Length: 153 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX31_RS20700 (LUX31_20700)	4694811..4695602	+	792	WP_235476281.1	helix-turn-helix transcriptional regulator	-
LUX31_RS20705 (LUX31_20705)	4696137..4697837	+	1701	WP_235476282.1	hypothetical protein	-
LUX31_RS20710 (LUX31_20710)	4697834..4698217	+	384	WP_235476283.1	hypothetical protein	-
LUX31_RS20715 (LUX31_20715)	4698260..4698670	-	411	WP_235476284.1	PIN domain-containing protein	-
LUX31_RS20720 (LUX31_20720)	4698667..4698888	-	222	WP_235476288.1	type II toxin-antitoxin system VapB family antitoxin	-
LUX31_RS20725 (LUX31_20725)	4699616..4700074	+	459	WP_235476289.1	helix-turn-helix domain-containing protein	Antitoxin
LUX31_RS20730 (LUX31_20730)	4700157..4700645	+	489	WP_235476296.1	PIN domain-containing protein	Toxin
LUX31_RS20735 (LUX31_20735)	4700743..4701069	-	327	WP_235476297.1	hypothetical protein	-
LUX31_RS20740 (LUX31_20740)	4701147..4702523	-	1377	WP_235476299.1	FtsK/SpoIIIE domain-containing protein	-
LUX31_RS20745 (LUX31_20745)	4702523..4702879	-	357	WP_235476301.1	hypothetical protein	-
LUX31_RS20750 (LUX31_20750)	4703049..4703837	-	789	WP_235476305.1	GntR family transcriptional regulator	-
LUX31_RS20755 (LUX31_20755)	4703945..4704352	+	408	WP_235479993.1	NUDIX hydrolase	-
LUX31_RS20760 (LUX31_20760)	4704399..4704827	+	429	WP_235476307.1	ATP-binding protein	-
LUX31_RS20765 (LUX31_20765)	4704821..4705228	+	408	WP_235476308.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (12-86)

Antitoxin

• SinI-like, DNA-binding domain (83-129)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17730.43 Da
• Isoelectric Point: 6.0920

>T228020 WP_235476296.1 NZ_CP089312:4700157-4700645 [Streptomyces sp. GQFP]
VASSKPSGPIKILDETFRNILKNHQDIPPEKLDRVRALMNAAIRDCLVRGHEPLIDAVKLPDPDDRHVLAAAIRAKAQVV
VTFNLKDFPTDALAPWDVEAVHPDAFLETQIDLAPQVVYGAVQRIADSWRKPPGTVDDVIARLEQQGLVVSAAALRTLAI
TP

Nucleotide

• Length: 489 bp

>T228020 NZ_CP123255:3506469-3506687 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 153 a.a.
• Molecular weight: 16547.94 Da
• Isoelectric Point: 5.6604

>AT228020 WP_235476289.1 NZ_CP089312:4699616-4700074 [Streptomyces sp. GQFP]
MAKTDIRPVKLPPEKAAAAAHALAQVRSYLAAHQDLAEITVTVEDGERELLALPREAVELLAGLLAHLGAGRAVSVVPSD
AELTTQQAADMLNVSRPFLIGLLEAGEIEYRTVGTHRRITASSLLQYKRKDDQRRREAADELTQLGQEMGMI

Nucleotide

• Length: 459 bp

>AT228020 NZ_CP123255:3506069-3506443 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG