Detailed information of TA system TA228017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4421978..4422998
• Replicon: chromosome
• Accession: NZ_CP089311
• Organism: Aureimonas altamirensis strain C2P003

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LUX29_RS21555
• Protein ID: WP_232415271.1
• Coordinates: 4422501..4422998 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LUX29_RS21550
• Protein ID: WP_232413032.1
• Coordinates: 4421978..4422421 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX29_RS21520 (LUX29_21520)	4417371..4419284	+	1914	WP_232413026.1	phage tail tape measure protein	-
LUX29_RS21525 (LUX29_21525)	4419281..4419601	+	321	WP_232413027.1	hypothetical protein	-
LUX29_RS21530 (LUX29_21530)	4419598..4420173	+	576	WP_232413028.1	phage tail protein	-
LUX29_RS21535 (LUX29_21535)	4420428..4421090	+	663	WP_232413029.1	hypothetical protein	-
LUX29_RS21540 (LUX29_21540)	4421087..4421515	+	429	WP_232413030.1	hypothetical protein	-
LUX29_RS21545 (LUX29_21545)	4421512..4421763	+	252	WP_232413031.1	hypothetical protein	-
LUX29_RS21550 (LUX29_21550)	4421978..4422421	+	444	WP_232413032.1	excisionase family DNA-binding protein	Antitoxin
LUX29_RS21555 (LUX29_21555)	4422501..4422998	+	498	WP_232415271.1	PIN domain-containing protein	Toxin
LUX29_RS21560 (LUX29_21560)	4423444..4423815	-	372	WP_232413033.1	thermonuclease family protein	-
LUX29_RS21565 (LUX29_21565)	4424082..4425323	-	1242	WP_232413034.1	porin	-
LUX29_RS21570 (LUX29_21570)	4425587..4426282	+	696	WP_232413035.1	hypothetical protein	-
LUX29_RS21575 (LUX29_21575)	4426279..4426656	-	378	WP_232413036.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4413086..4426282	13196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-89)

Antitoxin

• SinI-like, DNA-binding domain (79-125)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 19071.69 Da
• Isoelectric Point: 4.8117

>T228017 WP_232415271.1 NZ_CP089311:4422501-4422998 [Aureimonas altamirensis]
MASEGLYRAKWTDLIHDEWTRNLKKDRPDIDPHKIDRTRDLMNRAVLDCMVTGFEDLIPNLELPDPSDRHVLAAAIKCNA
DGIVTFNRKDFSTEIETQFGVEVQHPDDFLLAQLDLDEPKVITCLRECRSRLKNPPETAGEFLDRLGRQTLPKFVAQLRD
WESIL

Nucleotide

• Length: 498 bp

>T228017 NZ_CP123255:c2501545-2501369 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.62 Da
• Isoelectric Point: 6.4960

>AT228017 WP_232413032.1 NZ_CP089311:4421978-4422421 [Aureimonas altamirensis]
MSSDLLDRRGTYLPSDSEKELAEEASRALSRNSDSALRVKLDDGQELTLPVSAQRLLIHLLGEMSMGNAVTVIPVHAELT
TQQAASLLNVSRPYLISLLESGEIPFHKVGTHRRVAYKHLEAFKRKSHEERKSALDELAELTRDMGI

Nucleotide

• Length: 444 bp

>AT228017 NZ_CP123255:c2501323-2500907 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA