Detailed information of TA system TA228015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1298983..1299900
• Replicon: chromosome
• Accession: NZ_CP089310
• Organism: Bacillus velezensis strain A4P130

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: LUX28_RS06820
• Protein ID: WP_007407256.1
• Coordinates: 1299154..1299900 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LUX28_RS06815
• Protein ID: WP_003154807.1
• Coordinates: 1298983..1299153 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX28_RS06775 (1294194)	1294194..1295783	+	1590	WP_053573489.1	hypothetical protein	-
LUX28_RS06780 (1295796)	1295796..1296221	+	426	WP_053573490.1	hypothetical protein	-
LUX28_RS06785 (1296226)	1296226..1296423	+	198	WP_012117366.1	XkdX family protein	-
LUX28_RS06790 (1296480)	1296480..1297241	+	762	WP_053573491.1	hypothetical protein	-
LUX28_RS06795 (1297293)	1297293..1297556	+	264	WP_003154815.1	hemolysin XhlA family protein	-
LUX28_RS06800 (1297570)	1297570..1297833	+	264	WP_003154813.1	phage holin	-
LUX28_RS06805 (1297847)	1297847..1298725	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
LUX28_RS06815 (1298983)	1298983..1299153	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LUX28_RS06820 (1299154)	1299154..1299900	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LUX28_RS06825 (1300004)	1300004..1301002	-	999	WP_003154805.1	inorganic phosphate transporter	-
LUX28_RS06830 (1301015)	1301015..1301632	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LUX28_RS06835 (1301918)	1301918..1303234	-	1317	WP_007610842.1	amino acid permease	-
LUX28_RS06840 (1303557)	1303557..1304507	+	951	WP_031378808.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1264453..1335942	71489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T228015 WP_007407256.1 NZ_CP089310:c1299900-1299154 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T228015 NZ_CP123255:2052703-2052806 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT228015 WP_003154807.1 NZ_CP089310:c1299153-1298983 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT228015 NZ_CP123255:c2052844-2052573 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14