Detailed information of TA system TA228014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 521128..521765
• Replicon: chromosome
• Accession: NZ_CP089310
• Organism: Bacillus velezensis strain A4P130

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LUX28_RS02490
• Protein ID: WP_003156187.1
• Coordinates: 521415..521765 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LUX28_RS02485
• Protein ID: WP_003156188.1
• Coordinates: 521128..521409 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUX28_RS02465 (517493)	517493..518092	-	600	WP_053573675.1	rhomboid family intramembrane serine protease	-
LUX28_RS02470 (518185)	518185..518550	+	366	WP_015416872.1	holo-ACP synthase	-
LUX28_RS02475 (518715)	518715..519722	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
LUX28_RS02480 (519839)	519839..521008	+	1170	WP_053573676.1	alanine racemase	-
LUX28_RS02485 (521128)	521128..521409	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LUX28_RS02490 (521415)	521415..521765	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LUX28_RS02495 (521883)	521883..522704	+	822	WP_033575045.1	STAS domain-containing protein	-
LUX28_RS02500 (522709)	522709..523074	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
LUX28_RS02505 (523077)	523077..523478	+	402	WP_003156178.1	anti-sigma regulatory factor	-
LUX28_RS02510 (523490)	523490..524497	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
LUX28_RS02515 (524561)	524561..524890	+	330	WP_033575044.1	anti-sigma factor antagonist	-
LUX28_RS02520 (524887)	524887..525369	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
LUX28_RS02525 (525335)	525335..526123	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
LUX28_RS02530 (526123)	526123..526725	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T228014 WP_003156187.1 NZ_CP089310:521415-521765 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T228014 NZ_CP123255:1852561-1852815 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATTATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT228014 WP_003156188.1 NZ_CP089310:521128-521409 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT228014 NZ_CP123255:1852313-1852564 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5