Detailed information of TA system TA022801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3439488..3441059
• Replicon: chromosome
• Accession: NC_010554
• Organism: Proteus mirabilis HI4320

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B4F0T8
• Locus tag: PMI_RS15470
• Protein ID: WP_012368637.1
• Coordinates: 3439488..3440801 (-)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: PMI_RS15475
• Protein ID: WP_004249771.1
• Coordinates: 3440802..3441059 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMI_RS15450	3434657..3435460	+	804	WP_012368634.1	energy transducer TonB	-
PMI_RS15455	3435514..3437475	-	1962	WP_012368635.1	hypothetical protein	-
PMI_RS15460	3437507..3438436	-	930	WP_004246518.1	metallophosphoesterase	-
PMI_RS15465	3438621..3439085	-	465	WP_012368636.1	DUF1097 domain-containing protein	-
PMI_RS15470	3439488..3440801	-	1314	WP_012368637.1	type II toxin-antitoxin system HipA family toxin	Toxin
PMI_RS15475	3440802..3441059	-	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
PMI_RS15480	3441208..3442572	-	1365	WP_012368638.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
PMI_RS15485	3442710..3444350	-	1641	WP_004246512.1	membrane protein insertase YidC	-
PMI_RS19110	3444353..3444613	-	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
PMI_RS15490	3444577..3444936	-	360	WP_004246510.1	ribonuclease P protein component	-
PMI_RS15495	3444954..3445097	-	144	WP_004246509.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49670.78 Da
• Isoelectric Point: 6.5023

>T22801 WP_012368637.1 NC_010554:c3440801-3439488 [Proteus mirabilis HI4320]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T22801 NC_010554:c3440801-3439488 [Proteus mirabilis HI4320]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATGTGGGCATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTTTCGATGCCATTACAGCTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTGTTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGCGGCTATCGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGGATATCAAAAAAATAGAATATCAAAAACTCAATGATATTGAGCTTGAAAAAATTCTACTAGGATATA
AATCCAATACTCCACTCGGTATGCTTAAAGAATATACTGATTTCAGAATTTCCATCGCTGGAGCACAAGAAAAAACAGCA
TTACTTTTAAAAGATAATCATTGGTGTGTGCCACATAATAACACACCAACTACCCATATTATTAAACTGCCAATTGGTGT
TATTGAAAGTCATTCTTATACAATGGATTTATCCAATAGTGTAGAAAATGAGTATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCCGTCCCCCACTGCTCTATTATTAAAACAAAAAATGTTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TATTCATCTGATAAATCGTGGATAATACGTTTACCTCAAGAAGATTTTTGCCAAATTTTAAATATACCTTCTGCGCTAAA
ATATGAAAATGAAGGAGGGCCTGGCATTATCGATATTATGAAATATTTACTTGGCTCATCAAATGCCGATCAAGATCGTT
TTCATTTTATGAAGGCACAAGTGCTATTTTGGCTTTTGGCTGCAACGGATGGACACGCAAAGAATTTTTCTGTTTTTATC
AACCAAGAAGGACGTTTCCATCTCACTCCCCTTTATGACATTATGTCCATCTATCCCAATTTCGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTTGCTATGGGATTAAAAGCAAGCCGAGGCAAAAAATACGCCATAGAACAAATTTTTCCTCGTC
ATTTCTACCAAACAGCTGAAGCTGTCGGTTTCGATCCTTCATTAATGAGCGATATTCTTCGTTATTTTTATCAAGAAATG
GACGATGTCATACAACGCGTAAAACAGCAATTACCCGAGGATTTTCCTTCTGAGATTAGCAATACAATATTAGATGGAAT
GAAAGCACGCGCTGTTAGATTAGCTAACATTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT22801 WP_004249771.1 NC_010554:c3441059-3440802 [Proteus mirabilis HI4320]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT22801 NC_010554:c3441059-3440802 [Proteus mirabilis HI4320]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTGTCCAATTTTGAACTTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: B4F0T8

Antitoxin

• AlphaFold DB: B4F0T9