Detailed information of TA system TA228001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2679142..2679793
• Replicon: chromosome
• Accession: NZ_CP089307
• Organism: Lacticaseibacillus paracasei strain VHProbi F22

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K6S8L5
• Locus tag: MTX54_RS13160
• Protein ID: WP_003567661.1
• Coordinates: 2679142..2679525 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K0NCT7
• Locus tag: MTX54_RS13165
• Protein ID: WP_014566665.1
• Coordinates: 2679545..2679793 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX54_RS13155 (LT340_13155)	2678447..2678968	-	522	WP_012492007.1	QueT transporter family protein	-
MTX54_RS13160 (LT340_13160)	2679142..2679525	-	384	WP_003567661.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTX54_RS13165 (LT340_13165)	2679545..2679793	-	249	WP_014566665.1	antitoxin	Antitoxin
MTX54_RS13170 (LT340_13170)	2679861..2680997	-	1137	WP_003576478.1	alanine racemase	-
MTX54_RS13175 (LT340_13175)	2680984..2681358	-	375	WP_003567669.1	holo-ACP synthase	-
MTX54_RS13180 (LT340_13180)	2681528..2683036	-	1509	WP_003567670.1	DEAD/DEAH box helicase	-
MTX54_RS13185 (LT340_13185)	2683336..2684724	-	1389	WP_003567677.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13773.03 Da
• Isoelectric Point: 10.9764

>T228001 WP_003567661.1 NZ_CP089307:c2679525-2679142 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T228001 NZ_CP123251:c4443374-4443072 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8966.14 Da
• Isoelectric Point: 4.4610

>AT228001 WP_014566665.1 NZ_CP089307:c2679793-2679545 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEVYAH
IR

Nucleotide

• Length: 249 bp

>AT228001 NZ_CP123251:c4443075-4442791 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Structures

Toxin

• AlphaFold DB: K6S8L5

Antitoxin

• AlphaFold DB: K0NCT7