Detailed information of TA system TA022800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3156767..3157416
• Replicon: chromosome
• Accession: NC_010554
• Organism: Proteus mirabilis HI4320

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: PMI_RS14230
• Protein ID: WP_012368534.1
• Coordinates: 3156767..3157186 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: PMI_RS14235
• Protein ID: WP_012368535.1
• Coordinates: 3157183..3157416 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMI_RS14200	3152773..3153687	-	915	WP_004246811.1	fatty acid biosynthesis protein FabY	-
PMI_RS14205	3153711..3154148	-	438	WP_004246810.1	D-tyrosyl-tRNA(Tyr) deacylase	-
PMI_RS14210	3154229..3154846	-	618	WP_004246809.1	glucose-1-phosphatase	-
PMI_RS14215	3155188..3155496	-	309	WP_004246808.1	helix-turn-helix transcriptional regulator	-
PMI_RS14220	3155825..3156241	-	417	WP_004246806.1	hypothetical protein	-
PMI_RS14225	3156219..3156542	-	324	WP_012368533.1	hypothetical protein	-
PMI_RS14230	3156767..3157186	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
PMI_RS14235	3157183..3157416	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PMI_RS14240	3158166..3160001	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
PMI_RS14245	3160361..3161770	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T22800 WP_012368534.1 NC_010554:c3157186-3156767 [Proteus mirabilis HI4320]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T22800 NC_010554:c3157186-3156767 [Proteus mirabilis HI4320]
GTGAAAAAAGTCTATATGCTTGATACAAACATTTGTTCTTTTATCATGCGAGAGCAACCTATTTCTTTGCTCGAAAAACT
TCAGAAATGTGTAATGAACCACGATACTATTGTCATATCAGCGATTACTTATTCAGAGATGCGCTTTGGAGCAATAGGAA
AAAAAGCATCACCTAAACACAATCGTTTGGTTGATGCTTTCTGTGAACGCGTTGACGCGATTTTGGCATGGGATAAAGCG
GCGGTAGACGCAACTACCGTGATAAAAAAATGCTTATCTGATGTGGGATTGCCCATAGGAAATAATGATAGCGCGATTGC
TGGTCATGCTGTCGCGGTTAATGCAATATTGGTAACAAATAATACCCGTGAATTTTCACGAGTTGAAGGACTAAAAATAG
AGGACTGGACTCATGTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT22800 WP_012368535.1 NC_010554:c3157416-3157183 [Proteus mirabilis HI4320]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT22800 NC_010554:c3157416-3157183 [Proteus mirabilis HI4320]
ATGAGAACGAGAACTGTAGCTGTATTTAAAAACGGAAGTAACCGAGCCATTCGCTTACCTAAAGATTTCGATTTTGATGG
TGTCAATGAACTCGAGATAAGCCGAGATGGGGATTGTATTATTTTACGCCCTGTAAAACCTAACTGGATTTCATTACAAA
ACGAAGAAAAAGCCGACAGTGATTTCTTGCTTGAACGCGATGATGTTATTGAAGAAGGACGAGTTATTCTGTGA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0