Detailed information of TA system TA227999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 462502..463130
• Replicon: chromosome
• Accession: NZ_CP089307
• Organism: Lacticaseibacillus paracasei strain VHProbi F22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: MTX54_RS02270
• Protein ID: WP_012490993.1
• Coordinates: 462762..463130 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: MTX54_RS02265
• Protein ID: WP_003583264.1
• Coordinates: 462502..462762 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX54_RS02240 (LT340_02240)	458393..459136	+	744	WP_012490990.1	SDR family oxidoreductase	-
MTX54_RS02245 (LT340_02245)	459249..459875	+	627	WP_003577713.1	flavodoxin	-
MTX54_RS02250 (LT340_02250)	460126..460629	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
MTX54_RS02255 (LT340_02255)	460634..461695	+	1062	WP_003563374.1	ABC transporter permease	-
MTX54_RS02260 (LT340_02260)	461700..462389	+	690	WP_012490992.1	ABC transporter ATP-binding protein	-
MTX54_RS02265 (LT340_02265)	462502..462762	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
MTX54_RS02270 (LT340_02270)	462762..463130	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MTX54_RS02275 (LT340_02275)	463421..464791	-	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
MTX54_RS02280 (LT340_02280)	465155..465973	-	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
MTX54_RS02285 (LT340_02285)	466289..466558	-	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
MTX54_RS02290 (LT340_02290)	466799..467806	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T227999 WP_012490993.1 NZ_CP089307:c463130-462762 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T227999 NZ_CP123251:c4265248-4264949 [Providencia rettgeri]
ATGATATTTGATATTGTATTCGATGAGCGTGCTCTAAGGGAATGGAAAAAGCTTGATTACAGTATCCGAGAACAATTTAA
AAAGAAATTAAAGAAATTAAAGAAATTACAATTAAACCCATATATAGAGTCTCTGCGGCTACATGGTGAATTGTCTAATT
GCTATAAAATTAAATTAAGGGCATCAGGATTTCGCCTTGTGTACCAAGTTGTTGATGATGAAATTGTTATTTTTGTCGTT
GCCGTTGGCAAAAGAGAAGATAAAAAGGTTTATAACTCAGCGATAGAAAGGCTACCATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT227999 WP_003583264.1 NZ_CP089307:c462762-462502 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT227999 NZ_CP123251:c4265492-4265238 [Providencia rettgeri]
ATGAGTACAATACAAATTAGAGTTGATGAACAGCTGAAAAGAGATGCGTATCAAGCATTTGATAAATTAAATCTCTCGCC
TTCCGATGCACTAAGGCTATTTCTTCGTTATGTTGCAGAGAATGAAAAGCTCCCTTTTTCTGAAGTTTCTATTGTTGTGA
GCGAGACTGATGAGGATGAGGATATTTTAGCTGTTGTTCGTGAACGTTTAAAAAAGCCAGCTAAACGTATAAGAGTGAAC
ATTGATGATATTTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4