Detailed information of TA system TA227998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4086398..4087437
• Replicon: chromosome
• Accession: NZ_CP089304
• Organism: Pseudomonas sp. NIBR-H-19

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LS633_RS18950
• Protein ID: WP_210702224.1
• Coordinates: 4086398..4086973 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LS633_RS18955
• Protein ID: WP_210702223.1
• Coordinates: 4086970..4087437 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS633_RS18925 (LS633_18925)	4081731..4083719	+	1989	WP_210702227.1	heme lyase CcmF/NrfE family subunit	-
LS633_RS18930 (LS633_18930)	4083716..4084252	+	537	WP_150594234.1	DsbE family thiol:disulfide interchange protein	-
LS633_RS18935 (LS633_18935)	4084249..4084722	+	474	WP_210702226.1	cytochrome c-type biogenesis protein CcmH	-
LS633_RS18940 (LS633_18940)	4084715..4085914	+	1200	WP_232391629.1	c-type cytochrome biogenesis protein CcmI	-
LS633_RS18945 (LS633_18945)	4085939..4086346	+	408	WP_232391631.1	hypothetical protein	-
LS633_RS18950 (LS633_18950)	4086398..4086973	-	576	WP_210702224.1	PIN domain-containing protein	Toxin
LS633_RS18955 (LS633_18955)	4086970..4087437	-	468	WP_210702223.1	helix-turn-helix domain-containing protein	Antitoxin
LS633_RS18960 (LS633_18960)	4087589..4088776	-	1188	WP_042558268.1	MFS transporter	-
LS633_RS18965 (LS633_18965)	4089221..4090219	-	999	WP_042558269.1	sulfate ABC transporter substrate-binding protein	-
LS633_RS18970 (LS633_18970)	4090320..4091144	-	825	WP_042558270.1	ion transporter	-
LS633_RS18975 (LS633_18975)	4091166..4092080	-	915	WP_210702221.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21804.12 Da
• Isoelectric Point: 6.3324

>T227998 WP_210702224.1 NZ_CP089304:c4086973-4086398 [Pseudomonas sp. NIBR-H-19]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGRFRARWTKEIHCEWKRNLLKNRTDLTMEQLDRTSDLMDRAIPEACVIDY
EDLVTGLTLPDRDDRHVLAAAIRCGAGVIVTFNLKEFPDVLLAPFGIEAQHPDEFVENLFHLDPSAVVAAAQRQRQQLKN
PVMDIESFLNLLQRQGLPRSVQALASYRAIL

Nucleotide

• Length: 576 bp

>T227998 NZ_CP123251:c4195576-4195208 [Providencia rettgeri]
GTGGCGTTCTATAAAATTTCCTCATTTAAGCGAAGCACCAAAAAAATTAAGATAATGGATAAACACTTGCTTAAGGCTAC
AAATGAAGTTTTAGCGGGGGTTTATGAAGCAGACTTAGGAAGTGGTGTAATTAAAAAAAGGATTGCCATTAATGGAAAAG
GGAAAAGTGGTGGGGTCAGGGTTATTATATTCTTCAAAGTTAGCCATCATCTCTTTTTTGCCGATGGTTGGGAGAAATGC
AATATAGCTGCTAAAGGAGTAAAAGAAATTGAAGATGATGAACTAGATGCGTATAAGAATTTATCTCGACTTTTTCTTAA
TTATACGGATGATAAAATTGCTGATTTAGTTTTACATAATATTTTGTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17292.85 Da
• Isoelectric Point: 6.2292

>AT227998 WP_210702223.1 NZ_CP089304:c4087437-4086970 [Pseudomonas sp. NIBR-H-19]
MSIVIQPAINLPVEREVKAAVEGKRALAAYLATQFETQHIQIFDEQNEAHLVELPTSALRLLVDILAALAEGDAVKVVPI
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKTRRDTASEQAMKLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT227998 NZ_CP123251:c4195197-4194868 [Providencia rettgeri]
ATGAGCAATAAACTAGAAAACATGCTTGAGCTAGCGAAAGAGTTACATCAGGTTGATGCTGCACCAAAAAGTTTAGTTAC
TGATTTAGAACAGAAGGTTGGCAATCGTAAAATAAATAATAAAATCAAGGTAGTACCTATGATGAGCGGTGCGCAAATCA
AACAACTCCGTGCTCAATATGGAATGTCACAAGCCATGTTAGCTTTAGCGCTGGGCATGTCTAAGGAAAGCGTATCTAAG
TGGGAAAGAGAAGAAAAAAAACCCAGTGGCCCAGCTCTAAGAATGCTACATCTTATAGAGCAGCGAGGGCCAGAGATTTT
AATTATTTAA