Detailed information of TA system TA227997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 3695769..3696316
• Replicon: chromosome
• Accession: NZ_CP089304
• Organism: Pseudomonas sp. NIBR-H-19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: LS633_RS17090
• Protein ID: WP_016502317.1
• Coordinates: 3695769..3696044 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: LS633_RS17095
• Protein ID: WP_016502318.1
• Coordinates: 3696044..3696316 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS633_RS17075 (LS633_17075)	3691814..3693379	+	1566	WP_047283017.1	PAS domain-containing methyl-accepting chemotaxis protein	-
LS633_RS17080 (LS633_17080)	3693416..3695146	+	1731	WP_232391552.1	EAL domain-containing protein	-
LS633_RS17085 (LS633_17085)	3695356..3695625	+	270	WP_011077892.1	hypothetical protein	-
LS633_RS17090 (LS633_17090)	3695769..3696044	-	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LS633_RS17095 (LS633_17095)	3696044..3696316	-	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
LS633_RS17100 (LS633_17100)	3696330..3696743	-	414	WP_016502319.1	hypothetical protein	-
LS633_RS17105 (LS633_17105)	3696750..3697490	-	741	WP_232391553.1	DUF2807 domain-containing protein	-
LS633_RS17110 (LS633_17110)	3697744..3698178	+	435	WP_126624766.1	hypothetical protein	-
LS633_RS17115 (LS633_17115)	3698261..3698821	+	561	WP_048388137.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3677033..3714673	37640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T227997 WP_016502317.1 NZ_CP089304:c3696044-3695769 [Pseudomonas sp. NIBR-H-19]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T227997 NZ_CP123251:2949487-2949795 [Providencia rettgeri]
ATGCAATATTGCCTCTATCAAAACCGAGAAGATACGGTCAAATACCCATATTTACTGGATATTCAAAGTAACATTATTGA
TATATTGAATACTCGTTTAGTCATCCCGTTATTCGATAGCAGGCTAGTTAAAAAGCCATTACCTGTACGCTTAAATCCAC
AATTATCAATCAACGGGCAGGTTTTTATTTTAATGACTCACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTAAGTAGCCAGCGAGACACCATTAAACACGCAATTGACTTACTGATTGATGGCTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT227997 WP_016502318.1 NZ_CP089304:c3696316-3696044 [Pseudomonas sp. NIBR-H-19]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT227997 NZ_CP123251:2949260-2949487 [Providencia rettgeri]
ATGAACAGAACAAAAAAAACCGTCAGTGTCACTGTCGCGCCTGAACTCTACGAACAAGCAAGGCGGGCTAAACTTAACTT
ATCAGCAATACTGGCTTGTGCCTTACAAGCTGAATTAAAACGCCTCGAAGCGCAAAAATGGAAAAATGACAATGTCGAAG
GCTTTAATGAGTTAAACAGGATAAATGAAGAATATGGATTACTCTCAGATAAATACAAGGATTTTTAA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38