Detailed information of TA system TA227994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1389110..1389635
• Replicon: chromosome
• Accession: NZ_CP089304
• Organism: Pseudomonas sp. NIBR-H-19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LS633_RS06580
• Protein ID: WP_210704192.1
• Coordinates: 1389339..1389635 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4R7UU95
• Locus tag: LS633_RS06575
• Protein ID: WP_016984130.1
• Coordinates: 1389110..1389349 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LS633_RS06565 (LS633_06565)	1384567..1387635	-	3069	WP_232392573.1	helicase-related protein	-
LS633_RS06570 (LS633_06570)	1387635..1388564	-	930	WP_232392574.1	WYL domain-containing protein	-
LS633_RS06575 (LS633_06575)	1389110..1389349	+	240	WP_016984130.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LS633_RS06580 (LS633_06580)	1389339..1389635	+	297	WP_210704192.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LS633_RS06585 (LS633_06585)	1389690..1390502	-	813	WP_210704193.1	DUF2135 domain-containing protein	-
LS633_RS06590 (LS633_06590)	1390506..1392125	-	1620	WP_210704194.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1362833..1389635	26802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11632.50 Da
• Isoelectric Point: 9.3782

>T227994 WP_210704192.1 NZ_CP089304:1389339-1389635 [Pseudomonas sp. NIBR-H-19]
MTYNLEFDHRALKEWNKLGDTVRHQFKKKLTEVLENPRIEANRLRELPDCYKVKLKSAGYRLIYQVLDQEIVVFVIAIGK
REREAAYEVAQDRLSLLP

Nucleotide

• Length: 297 bp

>T227994 NZ_CP123251:1532449-1532859 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACCCAACTGTTCTCAACCTGTGTTCATGGGGTCATTGGGAT
GTTTTTACTATTGGCTCCATGGTCCCCTGGTAACTCAATGATATGGTTGCCGCTATTAGTGGTTGTCGTTGCGAGTTGGG
CAAAGAGCCAGAAAAATATCAGTAAAATAAAAGGGGTTGCTGTACTGGTTAATGGCAATAAGGTTCAGTGGAAGAAAAAT
GAATGGAGAATACTGAAAGCACCTTGGCTTACCCGCTATGGTATATTACTGACACTAGAAGCGTTACAAGGTAAACCACA
AAAGCTCCATTTGTGGGTTGCAAAAGACGCATTGTCGGAAGAGAATTGGCGTAATTTAAACCAATTATTGCTGCAATACC
CTGATATTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8865.24 Da
• Isoelectric Point: 4.6017

>AT227994 WP_016984130.1 NZ_CP089304:1389110-1389349 [Pseudomonas sp. NIBR-H-19]
MASINIRIDDDLKQRSFAELEKLGVTPSELLRQTLQYVAERGKLPFKAALLSEEDEVLIAVVTERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT227994 NZ_CP123251:1532202-1532468 [Providencia rettgeri]
ATGGATATTGAGAATAAAGCGCGAATTCATTGGGCATGCCGCCGTGGTATGCGTGAATTAGATATTTCAATCATGCCGTT
TTTTGAGTATGAATATGATGACCTAACAACTCACGATAAGCAGGTATTTGTCCGTTTATTAGAATGTGCGGACCCTGATT
TATTCAATTGGCTAATGAATCACGGTCGTCCAGATGATGACGAACTTTTTCGTATGATTAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A4R7UU95