Detailed information of TA system TA227992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1396253..1397106
• Replicon: chromosome
• Accession: NZ_CP089302
• Organism: Bifidobacterium longum strain VHProbi Y08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V6XWY1
• Locus tag: MTX55_RS06300
• Protein ID: WP_007054468.1
• Coordinates: 1396253..1396744 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V6XX41
• Locus tag: MTX55_RS06305
• Protein ID: WP_008783109.1
• Coordinates: 1396744..1397106 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX55_RS06280 (LT344_06280)	1391325..1392557	+	1233	WP_207436794.1	histidine kinase	-
MTX55_RS06285 (LT344_06285)	1392592..1393317	+	726	WP_007056206.1	response regulator transcription factor	-
MTX55_RS06290 (LT344_06290)	1393357..1393998	+	642	WP_007052627.1	GNAT family N-acetyltransferase	-
MTX55_RS06295 (LT344_06295)	1394088..1396193	+	2106	WP_032747025.1	DUF262 domain-containing protein	-
MTX55_RS06300 (LT344_06300)	1396253..1396744	-	492	WP_007054468.1	PIN domain-containing protein	Toxin
MTX55_RS06305 (LT344_06305)	1396744..1397106	-	363	WP_008783109.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MTX55_RS06310 (LT344_06310)	1397252..1399951	-	2700	WP_007054466.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-141)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-53)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18328.91 Da
• Isoelectric Point: 4.5590

>T227992 WP_007054468.1 NZ_CP089302:c1396744-1396253 [Bifidobacterium longum]
MTDVSMQRILLDTNVLLDYLLHRDDHAKMAEAVMELGAKNNITLLCASLSLKDIAYLSSSAIRREFKPNESEVENFTRGF
LSSRVPWRCIEQVKEMCDIVAVDESTCDKAFSLQKRHRDFEDDLIIVAAQQSGANCVVTSDAELISHFPEYCKTPAEIVA
ALE

Nucleotide

• Length: 492 bp

>T227992 NZ_CP123246:61054-61452 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13974.03 Da
• Isoelectric Point: 5.0733

>AT227992 WP_008783109.1 NZ_CP089302:c1397106-1396744 [Bifidobacterium longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDKMYDEQLDAYYNMKRAD

Nucleotide

• Length: 363 bp

>AT227992 NZ_CP123246:60827-61054 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: V6XWY1

Antitoxin

• AlphaFold DB: V6XX41