Detailed information of TA system TA022799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3135345..3136044
• Replicon: chromosome
• Accession: NC_010554
• Organism: Proteus mirabilis HI4320

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: PMI_RS14115
• Protein ID: WP_004249093.1
• Coordinates: 3135345..3135731 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: PMI_RS14120
• Protein ID: WP_004246828.1
• Coordinates: 3135724..3136044 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMI_RS14100	3131364..3131945	-	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-
PMI_RS14105	3132196..3133101	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
PMI_RS14110	3133111..3135183	+	2073	WP_012368525.1	glycine--tRNA ligase subunit beta	-
PMI_RS14115	3135345..3135731	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PMI_RS14120	3135724..3136044	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
PMI_RS14125	3136093..3136527	-	435	WP_004246827.1	hypothetical protein	-
PMI_RS14130	3136520..3137404	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
PMI_RS14135	3137621..3138907	-	1287	WP_012368527.1	DUF3748 domain-containing protein	-
PMI_RS14140	3139044..3139661	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
PMI_RS14145	3139962..3140585	+	624	WP_004246821.1	guanylate kinase	-
PMI_RS14150	3140640..3140915	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T22799 WP_004249093.1 NC_010554:3135345-3135731 [Proteus mirabilis HI4320]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T22799 NC_010554:3135345-3135731 [Proteus mirabilis HI4320]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATAGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAAATATGACTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT22799 WP_004246828.1 NC_010554:3135724-3136044 [Proteus mirabilis HI4320]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT22799 NC_010554:3135724-3136044 [Proteus mirabilis HI4320]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAAGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTGCATTTAATAGATAAAAAAATATTATTCATCTA
G

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97