Detailed information of TA system TA227988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2440771..2441288
• Replicon: chromosome
• Accession: NZ_CP089301
• Organism: Novosphingobium kaempferiae strain Sx8-5

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LO787_RS10980
• Protein ID: WP_232495871.1
• Coordinates: 2440771..2441016 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LO787_RS10985
• Protein ID: WP_232495872.1
• Coordinates: 2441013..2441288 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO787_RS10970	2436821..2438542	-	1722	WP_232495869.1	malto-oligosyltrehalose trehalohydrolase	-
LO787_RS10975	2438542..2440650	-	2109	WP_232495870.1	glycogen debranching protein GlgX	-
LO787_RS10980	2440771..2441016	+	246	WP_232495871.1	BrnT family toxin	Toxin
LO787_RS10985	2441013..2441288	+	276	WP_232495872.1	BrnA antitoxin family protein	Antitoxin
LO787_RS10990	2441499..2442344	-	846	WP_232495873.1	ferredoxin--NADP reductase	-
LO787_RS10995	2442375..2442809	-	435	WP_232495874.1	hypothetical protein	-
LO787_RS11000	2442824..2443750	-	927	WP_232495875.1	hypothetical protein	-
LO787_RS11005	2443932..2444249	-	318	WP_232495876.1	hypothetical protein	-
LO787_RS11010	2444246..2444674	-	429	WP_232495877.1	DUF983 domain-containing protein	-
LO787_RS11015	2444776..2446128	+	1353	WP_232495878.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (16-87)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8934.40 Da
• Isoelectric Point: 8.6831

>T227988 WP_232495871.1 NZ_CP089301:2440771-2441016 [Novosphingobium kaempferiae]
MIIVWDEPKRIANLAKHGIDFADVTEEFFASALIRQAKDGRYAAIGRMDGVIAVIFMTLGTEGVSLVSARPASTKERRLI
Q

Nucleotide

• Length: 246 bp

>T227988 NZ_CP123244:c4321191-4321018 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTATCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10080.33 Da
• Isoelectric Point: 7.2033

>AT227988 WP_232495872.1 NZ_CP089301:2441013-2441288 [Novosphingobium kaempferiae]
VSKPKYTQADMDAVSDNPEWSEEDFAKAVPFAEAFPDLAKTIRRRGVQKAPTKVSTTIRLSPEVIAHFRSTGPGWQARID
DALKDWVAAHG

Nucleotide

• Length: 276 bp

>AT227988 NZ_CP123244:c4321515-4321219 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTGAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG