Detailed information of TA system TA227987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1154953..1156453
• Replicon: chromosome
• Accession: NZ_CP089301
• Organism: Novosphingobium kaempferiae strain Sx8-5

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LO787_RS05290
• Protein ID: WP_232494806.1
• Coordinates: 1155560..1156453 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LO787_RS05285
• Protein ID: WP_232494805.1
• Coordinates: 1154953..1155567 (+)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO787_RS05270	1151167..1152687	-	1521	WP_232494802.1	ATP-binding protein	-
LO787_RS05275	1152677..1154341	-	1665	WP_232494803.1	DNA cytosine methyltransferase	-
LO787_RS05280	1154365..1154826	+	462	WP_232494804.1	DNA mismatch endonuclease Vsr	-
LO787_RS05285	1154953..1155567	+	615	WP_232494805.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
LO787_RS05290	1155560..1156453	+	894	WP_232494806.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LO787_RS05295	1156450..1157754	-	1305	WP_232494807.1	hypothetical protein	-
LO787_RS05300	1157754..1159892	-	2139	WP_232494808.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1151167..1205620	54453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-259)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (6-91)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 33156.91 Da
• Isoelectric Point: 8.0347

>T227987 WP_232494806.1 NZ_CP089301:1155560-1156453 [Novosphingobium kaempferiae]
MAEPKSASAASIKQRLLNIARRSERPYDVVLVRYALERLLYRLSISAERDRFVLKGGMLVTLWIEGGHRETRDADFLGHG
EAGSEHVRRVFEGIMSIDVNDGLTFDTANIRVGPIRDESEYSGIRLRTTAHLERTRIPVTIDIGFGDALSSEAERLDYPT
LLDMGVPRIRTYPPAAVLAEKFQAIVALGIINGRMKDYFDLWAVPQTRLIDDEELDAAVKATFLRRRTPVPDKRPPGLSE
PFAIDPAKEAQWQAYARSVQLVGLSLQAVTDEIWKVFGPTCARVAAGSRKDSPKNTP

Nucleotide

• Length: 894 bp

>T227987 NZ_CP123244:c4223674-4223324 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCAGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 22397.76 Da
• Isoelectric Point: 10.1165

>AT227987 WP_232494805.1 NZ_CP089301:1154953-1155567 [Novosphingobium kaempferiae]
MTDSNSERDKLRRIVAATPIVRHYELRHAGIAASTIARATRDGELIRISRGLYQRADGEVDAEQSLAEAAKLVPRGVIAM
TSALAFHGLTDQMPRAVWVAIRAADWAPVPTYPPIRIIKFDDRYMQQGIEQHVISGVNVPIFSVTKALADVFRNKLVDRS
VAIEALRAALEQRKTTPAAIAQAATEGGAWKKMRPYLEALTADG

Nucleotide

• Length: 615 bp

>AT227987 NZ_CP123244:c4223919-4223668 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA