227983

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2187274..2187911 Replicon chromosome
Accession NZ_CP089287
Organism Bacillus velezensis strain GSBZ09

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag LT232_RS10665 Protein ID WP_003156187.1
Coordinates 2187274..2187624 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag LT232_RS10670 Protein ID WP_003156188.1
Coordinates 2187630..2187911 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT232_RS10625 (2182314) 2182314..2182916 - 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -
LT232_RS10630 (2182916) 2182916..2183704 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
LT232_RS10635 (2183670) 2183670..2184152 - 483 WP_007609591.1 anti-sigma B factor RsbW -
LT232_RS10640 (2184149) 2184149..2184478 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
LT232_RS10645 (2184542) 2184542..2185549 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
LT232_RS10650 (2185561) 2185561..2185962 - 402 WP_003156178.1 anti-sigma regulatory factor -
LT232_RS10655 (2185965) 2185965..2186330 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
LT232_RS10660 (2186335) 2186335..2187156 - 822 WP_003156182.1 STAS domain-containing protein -
LT232_RS10665 (2187274) 2187274..2187624 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LT232_RS10670 (2187630) 2187630..2187911 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
LT232_RS10675 (2188031) 2188031..2189200 - 1170 WP_234631072.1 alanine racemase -
LT232_RS10680 (2189317) 2189317..2190324 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
LT232_RS10685 (2190489) 2190489..2190854 - 366 WP_094031521.1 holo-ACP synthase -
LT232_RS10690 (2190947) 2190947..2191546 + 600 WP_015239195.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T227983 WP_003156187.1 NZ_CP089287:c2187624-2187274 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T227983 NZ_CP123244:2691905-2692012 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT227983 WP_003156188.1 NZ_CP089287:c2187911-2187630 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT227983 NZ_CP123244:c2691858-2691792 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References