Detailed information of TA system TA227982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1396463..1397380
• Replicon: chromosome
• Accession: NZ_CP089287
• Organism: Bacillus velezensis strain GSBZ09

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: LT232_RS06420
• Protein ID: WP_007407256.1
• Coordinates: 1396463..1397209 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LT232_RS06425
• Protein ID: WP_003154807.1
• Coordinates: 1397210..1397380 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT232_RS06400 (1391853)	1391853..1392803	-	951	WP_012117369.1	ring-cleaving dioxygenase	-
LT232_RS06405 (1393128)	1393128..1394444	+	1317	WP_007407254.1	amino acid permease	-
LT232_RS06410 (1394730)	1394730..1395347	+	618	WP_003154804.1	DUF47 domain-containing protein	-
LT232_RS06415 (1395360)	1395360..1396358	+	999	WP_007407255.1	inorganic phosphate transporter	-
LT232_RS06420 (1396463)	1396463..1397209	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LT232_RS06425 (1397210)	1397210..1397380	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LT232_RS06430 (1397477)	1397477..1397602	+	126	WP_023356844.1	hypothetical protein	-
LT232_RS06435 (1397637)	1397637..1398515	-	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
LT232_RS06440 (1398529)	1398529..1398792	-	264	WP_003154813.1	phage holin	-
LT232_RS06445 (1398806)	1398806..1399069	-	264	WP_003154815.1	hemolysin XhlA family protein	-
LT232_RS06450 (1399121)	1399121..1399882	-	762	WP_015239693.1	hypothetical protein	-
LT232_RS06455 (1399939)	1399939..1400136	-	198	WP_012117366.1	XkdX family protein	-
LT232_RS06460 (1400141)	1400141..1400512	-	372	WP_043021266.1	XkdW family protein	-
LT232_RS06465 (1400525)	1400525..1402156	-	1632	WP_052827325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T227982 WP_007407256.1 NZ_CP089287:1396463-1397209 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T227982 NZ_CP123244:c2506141-2505965 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT227982 WP_003154807.1 NZ_CP089287:1397210-1397380 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT227982 NZ_CP123244:c2505919-2505503 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14