Detailed information of TA system TA227979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 6015050..6015497
• Replicon: chromosome
• Accession: NZ_CP089282
• Organism: Pseudomonas amygdali pv. loropetali strain AAC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E7PTW5
• Locus tag: LT107_RS27160
• Protein ID: WP_004662083.1
• Coordinates: 6015050..6015322 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E7PTW6
• Locus tag: LT107_RS27165
• Protein ID: WP_003381808.1
• Coordinates: 6015312..6015497 (-)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT107_RS27130 (LT107_27130)	6010469..6011224	-	756	WP_057412162.1	IS21-like element ISPsy14 family helper ATPase IstB	-
LT107_RS27135 (LT107_27135)	6011245..6012759	-	1515	WP_057412163.1	IS21 family transposase	-
LT107_RS27140 (LT107_27140)	6013076..6013414	+	339	WP_243787582.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LT107_RS27145 (LT107_27145)	6013414..6013701	+	288	WP_005622028.1	XRE family transcriptional regulator	-
LT107_RS27150 (LT107_27150)	6013763..6014215	+	453	WP_243787581.1	TrfB-related DNA-binding protein	-
LT107_RS27155 (LT107_27155)	6014233..6014946	-	714	WP_248583754.1	YecA family protein	-
LT107_RS27160 (LT107_27160)	6015050..6015322	-	273	WP_004662083.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT107_RS27165 (LT107_27165)	6015312..6015497	-	186	WP_003381808.1	hypothetical protein	Antitoxin
LT107_RS27170 (LT107_27170)	6015548..6015826	-	279	WP_004667962.1	hypothetical protein	-
LT107_RS27175 (LT107_27175)	6015921..6016295	-	375	Protein_5356	helix-turn-helix domain-containing protein	-
LT107_RS27180 (LT107_27180)	6016475..6017683	-	1209	WP_248583755.1	IS91 family transposase	-
LT107_RS27185 (LT107_27185)	6017685..6017966	-	282	WP_248583756.1	hypothetical protein	-
LT107_RS27190 (LT107_27190)	6018278..6018547	+	270	Protein_5359	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	6003957..6017683	13726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10185.64 Da
• Isoelectric Point: 5.0749

>T227979 WP_004662083.1 NZ_CP089282:c6015322-6015050 [Pseudomonas amygdali pv. loropetali]
VLVEWLPQALEALEEIAGYLAERNPYAAEQMQATLEATVEALPLHPYIHRPGRVPGTREAVVHPNYLVVYRVGTERISIV
DVLHARQEYP

Nucleotide

• Length: 273 bp

>T227979 NZ_CP123244:1104442-1104777 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGATCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 7296.25 Da
• Isoelectric Point: 8.7721

>AT227979 WP_003381808.1 NZ_CP089282:c6015497-6015312 [Pseudomonas amygdali pv. loropetali]
MSIQLDPRVSEFETQEQADNYDRWFRLRIERSLADPRPPVPHDEAMARVRAMIEAKRRRAS

Nucleotide

• Length: 186 bp

>AT227979 NZ_CP123244:1104194-1104442 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0N8QF55

Antitoxin

• AlphaFold DB: A0A2V0QZQ1