Detailed information of TA system TA227977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2056228..2056741
• Replicon: chromosome
• Accession: NZ_CP089282
• Organism: Pseudomonas amygdali pv. loropetali strain AAC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LT107_RS09260
• Protein ID: WP_044322764.1
• Coordinates: 2056228..2056512 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q48KT0
• Locus tag: LT107_RS09265
• Protein ID: WP_011168200.1
• Coordinates: 2056502..2056741 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT107_RS09245 (LT107_09245)	2052380..2054308	+	1929	WP_243786641.1	methyl-accepting chemotaxis protein	-
LT107_RS09250 (LT107_09250)	2054339..2055271	-	933	WP_044320597.1	DMT family transporter	-
LT107_RS09255 (LT107_09255)	2055346..2056191	+	846	WP_243786642.1	LysR family transcriptional regulator	-
LT107_RS09260 (LT107_09260)	2056228..2056512	-	285	WP_044322764.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT107_RS09265 (LT107_09265)	2056502..2056741	-	240	WP_011168200.1	CopG family ribbon-helix-helix protein	Antitoxin
LT107_RS09270 (LT107_09270)	2056901..2057917	-	1017	WP_044320600.1	1-aminocyclopropane-1-carboxylate deaminase	-
LT107_RS09275 (LT107_09275)	2058104..2058610	+	507	WP_002552794.1	Lrp/AsnC family transcriptional regulator	-
LT107_RS09280 (LT107_09280)	2058760..2059668	+	909	WP_005744311.1	DUF808 domain-containing protein	-
LT107_RS09285 (LT107_09285)	2059847..2060632	-	786	WP_002552796.1	outer membrane protein OmpK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10635.26 Da
• Isoelectric Point: 10.4249

>T227977 WP_044322764.1 NZ_CP089282:c2056512-2056228 [Pseudomonas amygdali pv. loropetali]
MQVEWLKTALKNLDEEAAYIAQDNPAAAAAFVKAIQSSVTQLASFPAMGREGRIAGTREWPLPDLPYLTPYRIRSGRLQV
LRIFHTRRQSPPVW

Nucleotide

• Length: 285 bp

>T227977 NZ_CP123244:c814627-814250 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8688.78 Da
• Isoelectric Point: 4.4354

>AT227977 WP_011168200.1 NZ_CP089282:c2056741-2056502 [Pseudomonas amygdali pv. loropetali]
MSTMSLRLPDEMADTLAHLAKATGRSKSFLALDALREYLTREAWQIAEIQRAIEEADAGEFASTEDVKAVMDKWSGNAG

Nucleotide

• Length: 240 bp

>AT227977 NZ_CP123244:c815048-814674 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A3M6AYQ8