Detailed information of TA system TA227976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 646730..647484
• Replicon: chromosome
• Accession: NZ_CP089282
• Organism: Pseudomonas amygdali pv. loropetali strain AAC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LT107_RS02935
• Protein ID: WP_243787630.1
• Coordinates: 646730..647149 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LT107_RS02940
• Protein ID: WP_279578836.1
• Coordinates: 647146..647484 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT107_RS02930 (LT107_02930)	646057..646689	-	633	WP_248583770.1	recombinase family protein	-
LT107_RS02935 (LT107_02935)	646730..647149	-	420	WP_243787630.1	type II toxin-antitoxin system VapC family toxin	Toxin
LT107_RS02940 (LT107_02940)	647146..647484	-	339	WP_279578836.1	Arc family DNA-binding protein	Antitoxin
LT107_RS02945 (LT107_02945)	648152..648598	-	447	WP_054071054.1	GNAT family N-acetyltransferase	-
LT107_RS02950 (LT107_02950)	648665..650191	-	1527	WP_122321189.1	IS66 family transposase	-
LT107_RS02955 (LT107_02955)	650242..650577	-	336	WP_054070825.1	IS66 family insertion sequence element accessory protein TnpB	-
LT107_RS02960 (LT107_02960)	650574..650891	-	318	WP_005747240.1	transposase	-
LT107_RS02965 (LT107_02965)	650991..651794	+	804	Protein_577	IS3-like element IS51 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	585232..673239	88007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (32-73)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15137.49 Da
• Isoelectric Point: 5.1677

>T227976 WP_243787630.1 NZ_CP089282:c647149-646730 [Pseudomonas amygdali pv. loropetali]
MILLDTNVISEPQRRKPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T227976 NZ_CP123244:c636116-635652 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATACTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12603.42 Da
• Isoelectric Point: 8.6415

>AT227976 WP_279578836.1 NZ_CP089282:c647484-647146 [Pseudomonas amygdali pv. loropetali]
MDQSIILDLWYQKHIVIPLMAKTGNYQVANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLA
SIAREVGGLTDKEHTLFENTRITSPARAVSFE

Nucleotide

• Length: 339 bp

>AT227976 NZ_CP123244:c636451-636116 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA