Detailed information of TA system TA227974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 183522..184133
• Replicon: chromosome
• Accession: NZ_CP089282
• Organism: Pseudomonas amygdali pv. loropetali strain AAC

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: LT107_RS00810
• Protein ID: WP_096134620.1
• Coordinates: 183522..183872 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: LT107_RS00815
• Protein ID: WP_057431469.1
• Coordinates: 183885..184133 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT107_RS00795 (LT107_00795)	179115..180323	+	1209	WP_248583762.1	IS91 family transposase	-
LT107_RS00800 (LT107_00800)	180845..181825	+	981	WP_054991743.1	IS5-like element ISPsy2 family transposase	-
LT107_RS00805 (LT107_00805)	182891..183262	+	372	Protein_159	RHS repeat-associated core domain-containing protein	-
LT107_RS00810 (LT107_00810)	183522..183872	-	351	WP_096134620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LT107_RS00815 (LT107_00815)	183885..184133	-	249	WP_057431469.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LT107_RS00820 (LT107_00820)	184475..185437	+	963	WP_243787576.1	tyrosine-type recombinase/integrase	-
LT107_RS00825 (LT107_00825)	185607..185846	-	240	WP_057456425.1	hypothetical protein	-
LT107_RS00830 (LT107_00830)	186151..187131	-	981	WP_248583804.1	IS5-like element ISPsy2 family transposase	-
LT107_RS00835 (LT107_00835)	187174..188172	-	999	Protein_165	RHS domain-containing protein	-
LT107_RS00840 (LT107_00840)	188356..188538	-	183	WP_054070926.1	DUF6386 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	175321..187134	11813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12933.76 Da
• Isoelectric Point: 4.1953

>T227974 WP_096134620.1 NZ_CP089282:c183872-183522 [Pseudomonas amygdali pv. loropetali]
MNTFALRFTEVAQQSIEDQVEHLAITQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITDIAVLLVLGGKQSVEQALIRYCLLQPM

Nucleotide

• Length: 351 bp

>T227974 NZ_CP123244:205781-205888 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAACGGAAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9357.77 Da
• Isoelectric Point: 9.8452

>AT227974 WP_057431469.1 NZ_CP089282:c184133-183885 [Pseudomonas amygdali pv. loropetali]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDEAIALVKLLAISSREYVQGKHCSTDELKARLSRRFA
HK

Nucleotide

• Length: 249 bp

>AT227974 NZ_CP123244:c205732-205669 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT