Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 1771..2330 | Replicon | plasmid pO157 |
| Accession | NZ_CP089273 | ||
| Organism | Escherichia coli O157:H7 strain M1300706001A | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | LSI73_RS27690 | Protein ID | WP_000421248.1 |
| Coordinates | 2055..2330 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | LSI73_RS27685 | Protein ID | WP_001178089.1 |
| Coordinates | 1771..2055 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LSI73_RS27680 (1) | 1..858 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| LSI73_RS27685 (1771) | 1771..2055 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| LSI73_RS27690 (2055) | 2055..2330 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LSI73_RS27695 (2425) | 2425..2631 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| LSI73_RS27700 (2731) | 2731..2946 | - | 216 | Protein_5 | transposase | - |
| LSI73_RS27705 (2990) | 2990..3556 | - | 567 | Protein_6 | IS3-like element IS600 family transposase | - |
| LSI73_RS27710 (3556) | 3556..3972 | - | 417 | Protein_7 | IS91 family transposase | - |
| LSI73_RS27715 (3972) | 3972..4157 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| LSI73_RS27720 (4334) | 4334..6544 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| LSI73_RS27725 (6588) | 6588..6977 | + | 390 | WP_001172748.1 | cytochrome b562 | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | espP / stcE / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB | 1..94596 | 94596 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T227973 WP_000421248.1 NZ_CP089273:2055-2330 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T227973 NZ_CP123244:205298-205405 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT227973 WP_001178089.1 NZ_CP089273:1771-2055 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT227973 NZ_CP123244:c205249-205186 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |